Isolation,Identification,and Functional Analysis of Culturable Microorganisms in Cigar Tobacco Leaves from Various Regions
To clarify the species and functionality of culturable microorganisms in domestic cigar tobacco leaves,cured cigar tobacco leaves from Wuzhishan,Hainan Province(variety Haiyan 103)and and from Dazhou,Sichuan Province(variety Chuanxue No.1)were used as experimental materials.The culture-dependent method were employed to isolate microorganisms,which were subsequently identified through molecular sequencing.The enzyme and aromatic substances production abilities of the isolated microorganisms were investigated.The results showed the isolation of 52 different bacterial strains from Haiyan 103 and Chuanxue No.1 cigar leaves,belonging to five different families:Bacillaceae,Planococcaceae,Erwiniaceae,Enterobacteriales,and Moraxellaceae.Bacillus subtilis E-11,isolated from Hainan tobacco,exhibited the highest protease and pectinase activities of 85.02 U/mL and 256.71 U/mL,respectively.Additionally,four yeast strains were isolated from Hainan tobacco leaves,namely one strain from Pichia,one strain from Rhodotorula,and two strains from Debaryomyces.These yeast strains did not produce extracellular protease and pectinase,and Pichia sp.J-2 demonstrated the ability to metabolize 2-phenylethanol.The highest yield of 2-phenylethanol,333.81 mg/L,was observed after 72 h of fermentation.In conclusion,cigar tobacco contains a rich variety of culturable microbial resources.Bacillus subtilis E-11 and Pichia sp.J-2,isolated from Haiyan 103,show promising potential for degrading macromolecular substances and enhancing the aroma content in tobacco leaves.
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