Identification of HSF Gene Family in Eucommia ulmoides and Its Expression Analysis under Adverse Stresses
Heat shock transcription factors(HSFs)play important roles in plant growth and development and in response to abiotic stresses.In order to understand the information of HSF gene family members in Eucommia ulmoides and reveal the structural characteristics and expression patterns of EuHSFs,this study analyzed the physicochemical properties,protein structure,phylogeny,gene structure,conserved domains,promoter cis-acting elements,and the expression patterns of EuHSF genes in different developmental periods and abiotic stresses by bioinformatics methods and qRT-PCR.The results showed that a total of 21 EuHSF genes were identified from Eucommia ulmoides,which differed in protein properties,with amino acid numbers,protein theoretical molecular masses,isoelectric points and instability coefficients ranging from 68-369,7.72-42.06 ku,4.31-9.22,and 7.05-67.28,respectively,and mainly consisted of acidic,hydrophilic,and unstable nuclear proteins.Phylogenetic analysis showed that EuHSFs were divided into three subgroups,including ClassⅠ(1 EuHSF),ClassⅡ(7 EuHSFs)and ClassⅢ(13 EuHSFs),and promoter cis-acting element analysis revealed that a large number of light-responsive and hormone-responsive elements in EuHSF genes.In addition,EuHSF genes were expressed at different developmental periods in Eucommia ulmoides leaves,but there were significant differences in the expression patterns.qRT-PCR revealed that all EuHSFs responded to different abiotic stresses(high temperature,low temperature,high salinity,and drought),e.g.,most of the EuHSFs showed elevated expression under high-temperature stress with the prolongation of the treatment time,and they were strongly responsive to both high and low temperatures.In summary,the EuHSF gene family has the function of regulating plant response to abiotic stresses.
万方数据
维普
