Screening of Cellulose and Lignin Degrading Bacteria and Their Effect on Composting of Mushroom Residue
In order to screen high-efficiency composite bacteria that promote the degradation of cellulose and lignin in the composting process of mushroom residue,sodium carboxymethyl cellulose plate method,Congo red staining method and lignin plate method were used for preliminary screening,combined with filter paper strip disintegration test,aniline blue decolorization rate determination and cellulose and lignin enzyme activity determination test for rescreening from the mushroom residue waste,and then they were used for aerobic composting test to study the physical and chemical parameters and microbial community changes in the process,and to evaluate their degradation ability of cellulose and lignin in mushroom residue.The results showed that the enriched cellulose-degrading bacteria could completely degrade the filter paper within 6 days,and the activities of carboxymethyl cellulase(CMCase),filter paper enzyme(FPA),exoglucanase(C1)and β-glucosidase(β-Gase)reached 2.50,7.30,3.43 and 2.90 U/mL,respectively.The maximum decolorization rate of aniline blue reached 92.10%within 5 days by enriched lignin degrading bacteria,and the activities of lignin peroxidase(Lac),laccase(Lip)and manganese peroxidase(Mnp)reached 0.80,2.42 and 1.35 U/mL,respectively.The results of composting test showed that compared with the natural composting treatment(CK),the highest temperature of the pile body was 58.77℃,and the high temperature(>55℃)lasted for 5 days.The relative decomposition rate of cellulose and lignin increased by 35.83%and 54.33%respectively.The addition of microbial agents increased the richness of bacterial community in the middle stage of composting,and the relative abundance of Pseudomonas,Idiomarina,Glutamicibacter,Thiopseudomonas,Globicatella,Pusillimonas,Lysobacter and Brumimicrobium increased.Redundancy analysis(RDA)results showed that temperature,pH value and electrical conductivity(EC value)were the significant factors affecting the composition of bacterial community in the middle stage of composting.In summary,the composite degrading bacteria screened in this study can effectively degrade cellulose and lignin,and prolong the high temperature period of composting.
Degrading bacteriaCelluloseLigninScreeningMushroom residueComposting experimentBacterial community compositionEnvironmental factor
万方数据
维普
