In order to improve the selection efficiency of monoclonal antibodies and lower the preparation threshold of immunochromatographic strip,a universal hapten immunochromatographic strip was established by using biotin-labeled artificial antigen as a probe and avidin in a test line,and applied for selecting monoclonal antibodies against imidocarb(IM)and detecting IM residues.The results showed that IM artificial antigen and biotin probe were successfully prepared by 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide(EDC)method.Subsequently,monoclonal antibodies were prepared by immunizing mice and hybridoma cells technology,and the universal immunochromatographic strip was established.Using the strip,a monoclonal antibody cell line against IM with a titer of 1∶32 000 was successfully selected,and its sensitivity was up to 6.25 ng/mL.Based on the excellent monoclonal antibody and the universal strip,an immunochromatographic test strip for the detection of IM was established,and its sensitivity was 7 ng/mL in the milk.The immunochromatographic strip and its probe preparation method can be used in general hapten antibody screening,and a hapten immunochromatographic strip test can be established without professional research and development platform,which provides a more efficient method for antibody screening and a new technology for the development of immunochromatographic strip.
维普
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