Preparation of Universal Hapten Immunochromatographic Strip and Its Application in Imidocarb
In order to improve the selection efficiency of monoclonal antibodies and lower the preparation threshold of immunochromatographic strip,a universal hapten immunochromatographic strip was established by using biotin-labeled artificial antigen as a probe and avidin in a test line,and applied for selecting monoclonal antibodies against imidocarb(IM)and detecting IM residues.The results showed that IM artificial antigen and biotin probe were successfully prepared by 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide(EDC)method.Subsequently,monoclonal antibodies were prepared by immunizing mice and hybridoma cells technology,and the universal immunochromatographic strip was established.Using the strip,a monoclonal antibody cell line against IM with a titer of 1∶32 000 was successfully selected,and its sensitivity was up to 6.25 ng/mL.Based on the excellent monoclonal antibody and the universal strip,an immunochromatographic test strip for the detection of IM was established,and its sensitivity was 7 ng/mL in the milk.The immunochromatographic strip and its probe preparation method can be used in general hapten antibody screening,and a hapten immunochromatographic strip test can be established without professional research and development platform,which provides a more efficient method for antibody screening and a new technology for the development of immunochromatographic strip.
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