Morganella morganii is an opportunistic pathogen that can cause a variety of diseases and has become an important emerging pathogen worldwide.In order to explore the drug sensitivity and pathogenicity of Morganella morganii from swine,a suspected pathogenic strain was isolated from the intestines of dead pigs in Henan Province.Gram staining,matrix-assisted laser desorption/ionization time of flight mass spectrometry and 16S rRNA gene homology analysis were performed.Then the minimum inhibitory concentration of 14 drugs against the strain was determined,and the infection experiment was conducted in mice.The results showed that the bacterium was Gram-negative brevibacterium,which was identified as Morganella morgenii and named A185.The results of drug susceptibility test showed that the strain was resistant to amoxicillin,meropenem,tigacycline,polymyxin,doxycycline and cefoxitin,intermediate to ciprofloxacin and gentamicin,and sensitive to fosfomycin and ceftazidime.The results of infection experiment showed that the Kunming mice inoculated with Morganella morganii exhibited hemorrhage in liver,intestine and lung to varying degrees.The above results showed that the porcine Morganella morganii A185 had multi-drug resistance,and exhibited strong pathogenicity.
关键词
猪源摩氏摩根菌/分离鉴定/药敏试验/16S/rRNA/致病性
Key words
Porcine Morganella morganii/Isolation and identification/Drug sensitive test/16S rRNA/Pathogenicity
维普
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