Identification and Expression Analysis of PAL Gene Family in Tartary Buckwheat
Based on bioinformatics methods,the PAL gene family in tartary buckwheat was identified and analyzed at the whole genome level.The gene expression levels of PAL family in different tissues were detected using quantitative real-time PCR(qRT-PCR),so as to provide a reference for further investigation into the fundamental characteristics and gene functions of this gene family.The results showed that the PAL gene family in tartary buckwheat consisted of five members distributed across four chromosomes,with amino acids of encoding proteins ranged from 688 to 722 aa.All proteins exhibited similar physicochemical properties,including molecular weights ranging from 74 453.74 u to 78 377.58 u and isoelectric points ranging from 5.64 to 6.28.Furthermore,PAL members were stable and hydrophilic proteins,and were located in the cytoplasm.Collinearity analysis showed that there were six collinear gene pairs between tartary buckwheat PAL genes and soybean PAL genes,and three collinear gene pairs between tartary buckwheat PAL genes and alfalfa PAL genes.PAL gene family shared high protein sequence homology,with strictly conserved secondary structures and motifs.All PAL proteins contained a MIO(4-methylidene-imidazole-5-one)active group composed of Ala-Ser-Gly.PAL proteins from different species were relatively independent and conserved during evolution.PAL gene family member possessed many cis-acting elements regulating light response,hormone response,stress response and development in promoter.Expression pattern analysis results showed that five PAL genes were expressed in different degrees in tartary buckwheat tissues.Notably,the FtPAL2 gene showed specific and highly elevated expression levels in flower organs.
万方数据
维普
