Bioinformatics and Abiotic Stress Response of Tobacco NtTCHQD1
Glutathione S-transferase(GST)plays a crucial role in plant resistance to abiotic stress.To investigate the potential biological function of the NtTCHQD1 gene in the tetrachlorohydroquinone dehalogenase(TCHQD)subfamily in tobacco,with the aim of providing a novel gene resource for the development of tobacco resistance germplasm and variety selection,the biological information of NtTCHQD 1 was analyzed,and the tobacco variety K326 was used as material to be treated by nine kinds of stress,including water,temperature and hormone.The response characteristics of NtTCHQD1 gene in different treatments were analyzed by measuring the expression levels of NtTCHQD1 in different treatments.The results indicated that the NtTCHQD1 protein was an unstable,non-secretory,and hydrophilic protein with an instability coefficient of 40.52 and no signal peptide.The NtTCHQD1 protein mainly comprised serine,threonine,and tyrosine phosphorylation sites,which might be phosphorylated by these protein kinases,thereby participating in various physiological and biochemical processes of tobacco.The analysis of promoter cis-acting elements revealed that NtTCHQD1 contained hormone,anaerobic,drought,light,and other response elements,which might be involved in plant hormone signal transduction,light and temperature regulation,plant growth and development,and abiotic stress responses.The expression of NtTCHQD1 was elicited under diverse stress treatments,particularly in response to drought,high temperature,and low temperature.The responses of tobacco plants to various stresses exhibited tissue specificity,among which the response of roots to salicylic acid(SA)and gibberellin(GA3)was the most susceptible,and the response of leaves to other stress treatments except for SA and GA3 was the most vigorous.In conclusion,NtTCHQD1 might regulate the response of tobacco to hormone and abiotic stress during its growth and development,and could be employed as a novel gene resource for tobacco and other crops to breed outstanding varieties resistant to drought and extreme temperature.
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