Distinguishment of the Instars of Macrosiphoniella yomogicola in Artemisia argyi
The morphologic indicators of body length,body width,head width,antenna length,cornicle length,cauda length,and hind tibia length for Macrosiphoniella yomogicola at 1st,2nd,3rd,4th instars and adult were measured by stereoscopy,the data were analyzed by correlation analysis and regression analysis,and then the characteristics of Macrosiphoniella yomogicola at different instars were identified,to lay technical support for aphid integrated control in Artemisia argyi of Chinese medicinal materials.The results showed that morphologic indicators of Macrosiphoniella yomogicola had significant differences at different instars,and showed significantly positive correlation among them.Regression analysis indicated that head width,antenna length,cornicle length and hind tibia length of Macrosiphoniella yomogicola were linearly positively related to instars,but body length,body width and cauda length showed no linear significant correlation with instar.Further data analysis showed that cornicle length of wingless and winged Macrosiphoniella yomogicola displayed no overlap between adjacent instars,hind tibia length displayed no overlap between 1st and 2nd instar nymphs,antenna length displayed no overlap between 1st,2nd and 3rd instar nymphs,but body length,body width,head width and cauda length had overlap between adjacent instars.In conclusion,cornicle length can be screened as a main indicator for identification of instars of Macrosiphoniella yomogicola,and antennae length and hind tibia length can be as assistant indexes.