Identification and Expression Analysis of Auxin Response Factor(ARF)Gene Family in Tree Peony
To study the auxin response factor(ARF)gene family in tree peony,the gene family was identified and bioinformatics analysis was conducted.The results showed that a total of 19 ARF genes were identified in P.sufruticosa'Shima Nishiki',P.ostii and P.sufruticosa'Luoshen Xiaochun',and the ARF gene family members of tree peony were divided into four subfamilies based on the evolutionary classification of Arabidopsis thaliana ARF gene family,and according to the phylogenetic tree,there was a close evolutionary relationship between peony and Arabidopsis thaliana ARF homologous genes.There were differences in the length and isoelectric point of ARF proteins among different tree peony cultivars.All PsARFs contained conserved B3 and Auxin_resp domains,and most PsARFs also contained Aux/IAA domains.Conserved motif analysis showed that the number of Motif was 4-12,and gene structure analysis showed that the number of introns in the ARF gene of peony was 1-19,and there were some differences in the number of Motif and introns among different subfamilies.Tissue expression characteristics analysis showed that all PsARF genes were expressed in roots,stems and leaves of peony tissue culture seedlings,except that PsARF2b was not expressed in various tissues,but the expression levels in different tissues were very different.The expression levels of PsARF7,PsARF19,PsARF8a,PsARF8b,PsARF6,PsARF11a,PsARF 11b,PsARF9,PsARF 16b,PsARF17,PsARFx were the highest in roots,PsARF 5,PsARF 3 and PsARF 4 were the highest in stems,and PsARF2a,PsARF 1,PsARF16a and PsARF 10 were the highest in leaves.
万方数据
维普
