Expression Analysis of gga-miR-103-3p in Different Tissues and Cells in Chicken and Screening of Its Key Target Genes
In order to preliminarily elucidate the regulatory role of gga-miR-103-3p in fat deposition and muscle development in chickens,fluorescence quantitative PCR(qPCR)was used to detect the expression of gga-miR-103-3p in abdominal fat,liver,leg muscle,abdominal adipocytes,intramuscular adipocytes and adult myoblasts in chickens of the S3 lineage and recessive white-feathered(RR)chickens,and its key target genes were screened.The results showed that gga-miR-103-3p was expressed in liver,abdominal fat,leg muscle,abdominal adipocytes,intramuscular adipocytes and adult myoblasts,and there were differences among tissues and breed(line).In the liver,gga-miR-103-3p expression level was significantly higher in S3 line chickens than that in RR chickens at 0 week of age(0W).For S3 chickens,gga-miR-103-3p expression level was the highest at 16 weeks of age(16W),which was significantly higher than those at 0W,2 weeks of age(2W),8 weeks of age(8W)and 14 weeks of age(14W);For RR chickens,gga-miR-103-3p expression level was significantly higher at 16W and 14W than those at 0W and 8W.In abdominal fat,gga-miR-103-3p expression level was significantly higher in RR chickens than that in S3 chickens at 0W.gga-miR-103-3p expression level was significantly higher at 0W than those at 2W,8W,14W and 16W in S3 and RR chickens,and decreased in S3 chickens with the advancement of developmental period.In the leg muscle,the expression level of gga-miR-103-3p was significantly higher in RR chickens than that in S3 chickens at 16W,and vice versa at 0W;The expression level of gga-miR-103-3p in S3 chickens generally declined with the advancement of developmental period,and the expression level was significantly higher at 0W than those at other weeks of age;The expression level of gga-miR-103-3p in RR chickens first declined and then increased with the advancement of developmental period,and the expression level was significantly higher at 16W than those at other weeks of age.In abdominal adipocytes and intramuscular adipocytes,the expression levels of gga-miR-103-3p at 4 d of differentiation and 6 d of differentiation were significantly higher than that at the proliferative stage;In adult myoblasts,the expression level of gga-miR-103-3p increased with the extension of differentiation,and the expression levels of gga-miR-103-3p were significantly higher at 5 d of differentiation and 7 d of differentiation than that at the proliferative stage.The GO,KEGG enrichment and protein interaction analyses of gga-miR-103-3p target genes showed that FBXW7,CDK6,NF1 and PIK3R1 were important target genes of gga-miR-103-3p,of which FBXW7 and PIK3R1 were particularly critical.In conclusion,gga-miR-103-3p may affect muscle development and fat deposition through FBXW7,CDK6,NF1 and PIK3R1,of which FBXW7 and PIK3R1 are particularly critical.
万方数据
维普
