Identification of Rice B12D Gene Family and Expression Analysis under Submergence Stress
In order to better understand the function of rice B12D gene,the genome-wide identification and bioinformatics analysis of rice B12D gene family members were done,and the expression difference of B12D gene in rice(Nipponbare)and upland rice(Kunhan 1)under submergence stress was analyzed by real-time fluorescence quantitative PCR(qRT-PCR).Finally,the possible interaction proteins of B12D were found by Alphafold analysis.The results showed that there were six B12D genes in rice genome,which were distributed on three chromosomes.The number of amino acids of B12D protein ranged from 89 aa to 98 aa,and the isoelectric point ranged from 9.65 to 10.34,which was alkaline.Except that OsB12D3 was located in the cell membrane,the other five B12D proteins were located in mitochondria.The B12D gene family had only one pairs of collinearity genes between Arabidopsis thaliana and rice,and three pairs of collinearity genes between rice and maize.In addition,OsB12D1 gene and OsB12D2 genes had a collinearity relationship with OsB12D3 gene.The protein sequence of rice B12D family had high homology and strong conservation.The promoter of rice B12D gene family contained a large number of light response elements,hormone response elements,stress response elements and development related elements.The expression levels of six B12D genes were different in different tissues,OsB12D1 and OsB12D2 genes were mainly expressed in roots;OsB12D3 gene was mainly expressed in ovary,embryo and endosperm;OsB12D4 gene was highly expressed in most tissues;OsB12D5 and OsB12D6 genes were significantly expressed in stamens,the expression level of OsB12D5 gene was lower,the expression level of OsB12D6 gene was higher,and there were different expression levels in other tissues for OsB12D6 gene.The expression levels of OsB12D1 and OsB12D2 genes in Nipponbare were significantly higher than that in Kunhan 1,the increase of OsB12D1 and OsB12D2 gene expression levels after submergence stress was higher than that in Kunhan 1,and the decrease of ATP content was lower than that in Kunhan 1.Rice B12D interacted with heme C and cytochrome C1.In summary,the rice B12D gene family may play an important role in rice growth and development,response to submergence stress and so on.
RiceB12D gene familyIdentificationSubmergence stressExpression modeInteracting protein
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