摘要
为明确鲜烟叶成熟度的量化指标,以中烟100为试验材料,采收SPAD值分别为10～15(S10～15)、15～20(S15～20)、20～25(S20～25)的中部烟叶,研究不同SPAD值中部烟叶的代谢组学特征和烤后烟叶外观评价、物理特性、化学成分、香气含量以及感官质量.结果显示,S15～20处理烤后烟叶外观质量优,物理特性表现良好,化学成分协调,中性致香物质总量最高,成熟度高.通过偏最小二乘法判别分析法(PLS-DA),在2个对比组别中筛选出32种共有差异代谢物,包括L-色氨酸、γ-氨基丁酸、香叶酸、吡咯-2-羧酸、海藻糖、曲二糖、烟酸D-核糖核苷、1-甲基腺苷、鞘氨醇、美利比醇、吲哚、东莨菪碱、18-羟基皮质酮、25-羟基胆固醇和山柰苷等,这些差异代谢物可能是烟叶成熟过程中较关键的差异代谢物.经过进一步筛选,在2个组别中共筛选出48种显著差异代谢物,包括11种氨基酸、7种类固醇、6种有机酸、6种糖、3种醇、3种核苷酸、2种黄酮、1种维生素以及9种其他代谢物.这些显著差异代谢物主要富集在氨基酸的生物合成、ABC转运蛋白、花生四烯酸代谢、赖氨酸降解、苯丙烷类化合物的生物合成和丁酸代谢等通路中,这些通路可能在烟叶的成熟过程中起着重要的调控作用.
Abstract
To clarify the quantitative indicators of fresh tobacco leaf maturity,Zhongyan 100 was used as the experimental material.Mid-stem leaves with SPAD values of 10-15(S10～15),15-20(S15～20),and 20-25(S20～25)were harvested to explore the metabolomics characteristics,as well as the appearance evaluation,physical properties,chemical composition,aroma content,and sensory quality of their post-cured leaves.The results showed that the post-cured leaves of the S15～20 treatment obtained the optimal quality,including better appearance quality,good physical properties,harmonious chemical components,and the highest total amount of neutral aromatic substances,showing high maturity.According to the partial least squares discriminant analysis(PLS-DA),32 mutual differential metabolites were identified between two comparison groups,including L-tryptophan,gamma aminobutyric acid,geranic acid,pyrrole-2-carboxylic acid,trehalose,kojibiose,nicotinate D-ribonucleoside,1-methyladenosine,sphinganine,melibiitol,indole,scopoletin,18-hydroxycorticosterone,25-hydroxycholesterol,and kaempferide,et al.These differential metabolites might be key differential metabolites during the maturation process of tobacco.Then,a total of 48 significantly differential metabolites were identified across the two groups,including 11 amino acids,7 steroids,6 organic acids,6 sugars,3 alcohols,3 nucleotides,2 flavonoids,1 vitamin,and 9 other metabolites.These significantly differential metabolites were primarily enriched in pathways such as amino acid biosynthesis,ABC transporters,arachidonic acid metabolism,lysine degradation,biosynthesis of phenylpropanoids,and butanoate metabolism,which might play important regulatory roles during the maturation of tobacco leaves.
国家科技期刊平台
NSTL
万方数据