The Complete Gene Sequencing of Bacilus subtilis Isolated from Rehmannia glutinosa and Study of Genes Related to Growth-promoting Effects
To investigate the genes associated with the growth-promoting effects of Bacillus subtilis in Rehmannia glutinosa,whole genome sequencing was performed in this study and the genes associated with the growth-promoting effects were discovered through functional annotation.The results showed that the genome size of Bacillus subtilis,an endophytic growth-promoting bacterium of Rehmannia glutinosa,was 4 310 699 bp,with a GC content of 43.44%,and a total of 4 521 predicted coding genes.A total of 3 013,3 354,4 395,206,52,and 212 functional genes were annotated in the clusters of orthologous groups(COG),gene ontology(GO),kyoto encyclopedia of genes and genomes(KEGG),carbohydrate-active enzymes(CAZyme),comprehensive antibiotic resistance database(CARD),and virulence factor database(VFDB),respectively.The annotation results indicated that there were 46 genes related to growth-promoting function,with the majority of these genes involved in phosphorus solubilization,nitrogen fixation,and iron carrier processes.In the meantime,anti-SMASH predicted that a total of 409 genes were enriched into 12 secondary metabolite synthesis gene clusters,with the majority encoding eight repressors,including fengycin,1-carbapen-2-em-3-carboxylic acid,bacillaene,bacilysin,subtilosin A,surfactin,pulcherriminic acid,and bacillibactin.Among these,pulcherriminic acid and bacillibactin had been observed to facilitate the chelation of ferric ions in the surrounding environment.
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