Study on Genetic Diversity and DNA Fingerprint Map of Pumpkin Based on ISSR Markers
In order to provide theoretical basis for pumpkin variety identification,germplasm conservation and innovation,31 pumpkin germplasm resources were used as materials,and 7 inter-simple sequence repeat(ISSR)primers with rich polymorphic information were selected to analyze the genetic diversity of the materials and establish DNA fingerprint map.The results showed that a total of 73 bands were amplified by 7 ISSR primers,including 72 polymorphic bands(polymorphic band rate:98.63% ).The average number of observed alleles,the average number of effective alleles,the average Nei's gene diversity index,and the average Shannon's diversity information index were 1.986 3,1.570 4,0.329 9 and 0.494 4,respectively.The interspecific genetic similarity coefficients ranged from 0.12 to 0.89,with an average value of 0.46.Unweighted pair-group method with arithmetic means(UPGMA)clustering analysis showed that 31 pumpkin materials were classified into Cucurbita moschata D.,Cucurbita pepo L.and Cucurbita maxima D.at the genetic similarity coefficient of 0.46.Furthermore,the DNA fingerprint map for 31 pumpkin materials was constructed using the 14 polymorphic sites amplified by primer UBC811 and primer UBC843.All varieties in the DNA fingerprint map were unique,which could be used for molecular identification of varieties.In conclusion,the genetic diversity of 31 pumpkin materials was rich,Cucurbita moschata D.was closer to Cucurbita pepo L.,and the two were more distantly related to Cucurbita maxima D.,which could provide scientific basis for the conservation of pumpkin germplasm resources and variety innovation.The DNA fingerprint map of pumpkin based on the ISSR molecular marker technology could provide a new way for the identification of pumpkin varieties.
国家科技期刊平台
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