Analysis of Inhibitory Activity of Bacillus mojavensis against Gram-Positive Bacteria and Its Antibacterial Secondary Metabolites
In order to screen endophytes of medicinal plants with good antibacterial effect against gram-positive bacteria,surface disinfection method and plate confrontation method were used to isolate and screen endophytes of rosemary with antagonistic effect against Erysipelothrix rhusiopathiae,Streptococcus and Staphylococcus.Morphological and molecular biological identification of these endophytes were conducted,and their antibacterial active substances were explored through genome analysis.The results showed that seven strains of endophytic bacteria were isolated from different tissues such as roots,stems and leaves of rosemary.A gram-positive endophyte(HNYJJ2211C)with good antibacterial effect was obtained from seven endophyte strains by plate confrontation assay.It was identified as Bacillus mojavensis(GCF000245335.1)by morphology and molecular identification,which could effectively inhibit Erysipelothrix rhusiopathiae,Streptococcus and Staphylococcus.Whole genome sequencing analysis showed that the genome size of HNYJJ2211C was 4 147 805 bp,the content of G+C was 43.8%,and the number of its coding genes was predicted to be 4 075.3 311,1 453,2 503,440 and 158 genes were annotated by COG,GO,KEGG,VFDB and CAZy respectively.AntiSMASH software predicted that there were six gene clusters related to the synthesis of secondary metabolites.Surfactin,bacillaene,fengycin,bacillibactin,subtilosin A and bacilysin were the main secondary metabolites.In the HNYJJ2211C genome,a gene called TasA was discovered,located at 24 807 14-24 814 999 bp,having a total length of 786 bp(gene2481)and encoding 261 amino acids.From the gene clusters associated with antibacterial active substances contained in its genome,it can be predicted that this strain has the potential to develop new drugs and serve as a feedable microorganism.
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