Cloning and Function Analysis of Rice Sucrose Transporter OsSUT4 Gene
The rice sucrose transporter gene OsSUT4 was cloned,and sucrose transport capability of OsSUT4 was verified by yeast heterologous expression system.The gene was overexpressed in rice,the phenotype of transgenic plants was observed,and the salt tolerance and drought resistance was identified,so as to explore the role of the OsSUT4 gene in stress resistance in rice and provide new gene resources and theoretical foundations for rice genetic improvement.The results showed that OsSUT4 gene was primarily expressed in the flag leaves at jointing stage;The sucrose transport capability of OsSUT4 was confirmed for the first time using the yeast heterologous expression system;Overexpression of OsSUT4 gene did not significantly alter the grain morphology or the thousand-grain weight,but significantly increased plant height and survival rates under salt and drought stresses,thereby enhancing the salt tolerance and drought resistance of rice.
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