尿石素A对肾癌786-O细胞的作用机制研究
Biological effects of Urolithin A on renal carcinoma 786-O cells
张颖 1陈茜 1石华 2郭靖 1许金红 3徐述雄 4龚茂迪4
作者信息
- 1. 吉首大学医学院,吉首 416000
- 2. 铜仁市人民医院泌尿外科,铜仁 554300
- 3. 铜仁市人民医院内科,铜仁 554300
- 4. 贵州省人民医院泌尿外科,贵阳 550000
- 折叠
摘要
目的:研究尿石素A(urolithin A;UA)对肾癌 786-O细胞的生物学作用及相关机制.方法:利用Pubchem和SwisstargetPrediction数据库对尿石素A结构及靶点进行检索和分析,同时,CCK-8法、流式细胞术、Transwell迁移和侵袭实验分别检测UA对786-O细胞的增殖、凋亡、周期、细胞迁移和细胞侵袭的影响;Western blotting 法检测P62、凋亡相关蛋白caspase-3、自噬相关蛋白LC3、Bcl-2、Bax蛋白的表达水平.结果:UA的靶点预测大多为激酶和细胞周期蛋白,UA以浓度依赖的方式抑制了786-O细胞的增殖活性,抑制786-O细胞中p62、bcl2表达,促进caspase-3、bax、lc3-Ⅱ表达.UA细胞减弱了786-O细胞的侵袭和迁移能力.结论:UA对786-O细胞具有抑制增殖、侵袭和迁移以及诱导凋亡等生物学活性,其作用机制可能与UA抑制肾癌细胞中p62、bcl2表达,促进caspase3、bax、lc3表达有关.
Abstract
Objective To investigate the effect of UA on renal carcinoma 786-O cells and the potential mechanism.Methods Pubchem and Swiss target Prediction databases were used to search the structure and target of UA,and the promising targets of UA were picked out according to the probability of action marks,which ware greater than 0.The effects of UA on the viability,migration and invasion of 786-O cells were detected by CCK-8,flow cytometry,Transwell migration and invasion assay,respectively.The expression levels of P62,caspase3,lc3,Bcl-2 and Bax proteins were detected by Western blotting.Results Most of the predicted targets of UA were kinases and cyclins.UA inhibited the proliferation of 786-O cells in a concentration-dependent manner,as well as inhibited the expression of p62 and bcl2 in 786-O cells.And UA inhibited the migration and invasion ability.Moreover,the expression of caspase3,bax and lc3-Ⅱ were down-regulated in 786-O cells.Conclusion UA can inhibit proliferation,induce apoptosis,inhibit migration and invasion in renal carcinoma cells.The mechanism may be related to inhibiting p62,bcl2 expression and promoting caspase3,bax and lc3-Ⅱ expression in renal carcinoma cells.
关键词
尿石素A/肾癌/786-O细胞/增殖/周期/凋亡Key words
urolithin/renal cell carcinoma/786-O cells/viability/cell cycle/apoptosis引用本文复制引用
基金项目
贵州省科技支撑计划项目(黔科合支撑[2021]一般085)
贵州省卫生健康委科学技术基金(gzwjkj2020-1-113)
出版年
2024
NETL
NSTL
万方数据