Effect of berberine on NEK7/NLRP3 pathway in rat cerebral ischemia/reperfusion injured brain tissue
Objective To observe the effect of berberine on NEK7/NLRP3 signaling pathway in cerebral ischemia reper-fusion,and to explore the neuroprotective effect and mechanism of berberine in cerebral ischemia reperfusion injury.Methods A rat cerebral ischemia reperfusion injury model was established by the wire bolus method,and randomly divided into a sham operation group,an MCAO model group,a low-dose berberine group(25 mg/kg),a medium-dose berberine group(50 mg/kg),and a high-dose berberine group(100 mg/kg),and the area of cerebral infarction was determined by TTC staining,the morpho-logical changes of brain tissue were observed by HE staining,and the neuroprotective effects and mechanisms of berberine in cerebral ischemia reperfusion injury were measured by ELISA.The morphology of brain tissues was observed by HE staining,alteration,serum IL-1β and IL-18 were measured by ELISA,and the mRNA and protein expression levels of NEK7,NLRP3,and Caspase-1 in brain tissues were detected by RT-qPCR and Western-blot.Results The volume of cerebral infarction was reduced in berberine group compared with the model group,and the volume of cerebral infarction was significantly smaller in the middle and high dose berberine group than that in the MCAO group.HE staining showed that the damage of cerebral cells in the berberine group was reduced compared with that in the model group.Compared with the MCAO group,the expression levels of IL-1β and IL-18 in the serum of rats in the berberine group were significantly decreased,and the mRNA and protein expression of NEK7,NLRP3,and Caspase-1 in the brain tissues of the berberine group differed significantly from that of the model group.Conclusion Berberine can significantly reduce the volume of cerebral infarction,attenuate the inflammatory re-sponse,and improve the symptoms of neurological deficits after CIRI,and its mechanism may be related to the NEK7/NLRP3 pathway.
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