Preparation of a dual functional biofilm and its role in tendon repair
Objective Tendon adhesion and rupture are the main complications after tendon injury surgery.This article aims to develop a biofilm material that can simultaneously prevent tendon adhesion and promote tendon healing,reducing the complications after tendon injury surgery.Methods This study prepared a chitosan/silk fibroin(CS/SF)-sodium alginate(SA)composite biofilm,which is composed of a chitosan/silk fibroin layer and an alginate layer.The mechanical properties,hy-drophilicity and biodegradability of the membranes were examined in this study,The biological characteristics of the bilayer membrane were tested through in vitro cytology experiments.Results CS/SF membrane has strong toughness and strength,with CS/SF 8:2 membrane having the highest elongation at break(48.65%)and CS/SF 7:3 membrane having the highest tensile strength(21.19 MPa);The contact angle of CS/SF blend film is around 70o,indicating its good hydrophilicity;In vitro degrada-tion experiments showed that after 4 weeks of degradation,the residual mass of CS/SF blend membrane was 82%to 85%,and the residual degradation amount of SA membrane was 55%,indicating that the membrane can maintain overall structural in-tegrity within 4 weeks.In vitro cytology experiments showed that tendon cells can rapidly proliferate on the CS/SF membrane,indicating their promoting effect on tendon cell proliferation;The slow proliferation of fibroblasts on the SA membrane suggests their ability to inhibit the growth of fibroblasts.Conclusion The biofilm has strong mechanical properties,good hydrophilicity,and suitable biodegradability.It has dual functions of promoting tendon regeneration and inhibiting fiber adhesion formation,and has certain application prospects in tendon injury repair.
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