Effects of Sodium Hydrosulfide on DNA Methylation and Antioxidant System of Dendrobium officinale under High Light Stress
The effects of sodium hydrosulfide(NaHS,H2S donor)on the antioxidant system and the DNA methylation profile patterns of Dendrobium officinale under high light stress were studied in this paper.The results showed that 200μmol·L-1NaHS could significantly promote the enzyme activity of SOD,CAT and POD,reduce the content of MDA and relieve oxidative damage caused by high light stress on Dendrobium officinale.While high concentration NaHS (600μmol·L-1)increased the oxidative damage.The analysis of methylation sensitive amplified polymorphism(MSAP)showed that the level of DNA methylation of control group was highest(35.4％).Then the seedling were treated with 0,200,600μmo1· L-1NaHS,the level of DNA methylation was 29.6％,33％ and 28.9％,respectively.With different concentration of NaHS(0,200 and 600μmol· L-1),demethylation and methylation of DNA were 6.4％,7.7％,6.7％and 9.0％,8.1％,9.2％,respectively.Several variable MSAP profiles were isolated and sequenced.By Blast analysis,the relevant photosynthetic enzymes and transcription factor were found.
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