Analysis on DNA Methylation Diversity of Diploid and Autotetraploid of Stevia Rebaudiana Bertoni
The diploid and corresponding autotetraploid of Stevia rebaudiana Bertoni were used in this research.The methylation sensitive amplification polymorphism(MSAP)was carried out to study the levels and patterns of DNA methylation in different ploidy plants.The polymorphism of autotetraploid DNA methylation was 46.10%,while the diploid was 46.53%.In comparison with diploid,in the autotetraploid genomic DNA total methylated rate and full methylated rate were decreased,but hemimethylated rate was increased.In the autotetraploid genomic DNA methylation patterns,29.72% was changed,including 12.8% in the rate of hypermethylation,11.8% in the rate of demethylation and 5.12% in the rate of methine-methylattion.