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二倍体和同源四倍体甜叶菊的DNA甲基化差异分析

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以甜叶菊(Stevia rebaudiana Bertoni)二倍体和同源四倍体为材料,应用甲基化敏感扩增多态性(Methylation sensitive amplified polymorphism,MSAP)技术研究不同倍性植株基因组之间的DNA甲基化水平及其模式变化.四倍体和二倍体甜叶菊甲基化敏感扩增多态性分别为46.10%、46.53%,与二倍体相比较,四倍体基因组DNA的总甲基化率和全甲基化率均有所降低,而半甲基化率则有所升高;四倍体基因组DNA甲基化模式有29.72%(过甲基化为12.8%,去甲基化为11.81%,次甲基化为5.12%)发生了改变.
Analysis on DNA Methylation Diversity of Diploid and Autotetraploid of Stevia Rebaudiana Bertoni
The diploid and corresponding autotetraploid of Stevia rebaudiana Bertoni were used in this research.The methylation sensitive amplification polymorphism(MSAP)was carried out to study the levels and patterns of DNA methylation in different ploidy plants.The polymorphism of autotetraploid DNA methylation was 46.10%,while the diploid was 46.53%.In comparison with diploid,in the autotetraploid genomic DNA total methylated rate and full methylated rate were decreased,but hemimethylated rate was increased.In the autotetraploid genomic DNA methylation patterns,29.72% was changed,including 12.8% in the rate of hypermethylation,11.8% in the rate of demethylation and 5.12% in the rate of methine-methylattion.

Stevia rebaudiana BertoniDiploidAutotetralploidMethylation-sensitive amplified polymorphismMethylation diversity

杨岚、李红、向增旭

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南京农业大学园艺学院,江苏 南京 210095

甜叶菊 二倍体 同源四倍体 甲基敏感扩增多态性(MSAP) 甲基化差异

2013

核农学报
中国原子能农学会 中国农业科学院农产品加工研究所(前中国农业科学院原子能利用研究所)

核农学报

CSTPCDCSCD北大核心
影响因子:1.5
ISSN:1000-8551
年,卷(期):2013.27(8)
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