Analysis of Population Genetic Structure of Maize by SSR Marker and Heterotic Grouping
This study was carried out to analyze the genetic diversity of maize inbred lines and to study genetic structure of germplasm population in order to provide the theoretical basis for preparing dominant hybrid seed.Simple sequence repeat (SSR) analysis of the 70 loci distributed uniformly throughout the maize genome was used to detect genetic variation among 96 maize inbred lines and 6 testers.The population genetic structure was assessed by using Structure2.3.1 software.Setting subsets (K) for 1-9,using ΔK(k) to determine the most suitable value of K,and then to generate the Q-matrix.Seventy loci identified 213 polymorphic bands.The average number of allele per SSR locus was 3.610 with a range from 2 to 7.The polymorphism information content(PIC) for the SSR loci varied from0.216 to 0.846 with an average of 0.614.Genetic similarities among the materials in this study ranged from 0.282 to 0.882 with an average of 0.670.The inbred lines could be classified into 6 clusters by UPGMA (unweighted pair group method arithmetic average) which could be simplified into SS (LRC,PA,Reid) and NSS (PB,SPT,Lancaster) heterotic groups.Assessment of population structure showed that the most appropriate K =7,namely,there were 7 sub-groups in the target population.The defined population analysis of inbred lines provided the basis for selecting new hybridization combinations.
国家科技期刊平台
NSTL
万方数据
维普
