首页|番茄NBS-LRR抗病基因家族全基因组分析

番茄NBS-LRR抗病基因家族全基因组分析

扫码查看
原文链接
  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
  • 维普
NBS-LRR(nucleotide-binding site and leucine-rich-repeat)是植物中最大类抗病基因家族之一.番茄基因组测序完成为全基因组水平上分析NBS-LRR抗病基因家族提供了机遇.利用生物信息学方法对番茄NBS-LRR抗病基因家族成员数目进行鉴定,并对其染色体定位和系统发育关系进行了分析.随后,将番茄和马铃薯NBS-LRR抗病基因进行了比较基因组学分析.结果表明:番茄基因组共包括252个NBS-LRR抗病基因,分布于番茄12条染色体上;63.5％的基因成簇存在,大部分为串联重复;系统发育关系分析表明番茄CC-NBS-LRR(CNL)亚家族较其他亚家族扩展程度大;同线性分析发现番茄中共79个NBS-LRR抗病基因与马铃薯基因具有同源关系.结果将为番茄NBS-LRR抗病基因家族的深入研究提供依据,同时也为利用番茄NBS-LRR基因进行基因定位以及相关抗病基因克隆等奠定基础.
Genome-wide Analysis of NBS-LRR Resistance Genes in Tomato
NBS-LRR (Nucleotide-Binding Site and Leucine-Rich-Repeat) was one of the largest resistance gene families in plant species.The available data of whole tomato genome will provide opportunity for analyzing NBS-LRR resistance gene family at the genome level.The number of tomato NBS-LRR genes was identified by bioinformatics method.Further,these resistance genes were performed for chromosome distribution and phylogenetic relationship.Subsequently,comparison analysis of tomato NBS-LRR resistance genes and corresponding genes in potato was performed.The result showed a total of 252 NBS-LRR resistance genes were identified.These genes located widely on 12 chromosomes.Approximate 63.5％ NBS-LRR resistance genes appeared in clusters and most of them were in tandem duplication.Microsynteny analysis revealed that 79 NBS-LRR resistance genes from tomato have high homology with those from potato.The results will provide a basis for studying deeply NBS-LRR resistance genes in tomato,and give a platform for mapping and cloning of resistance genes in future.

TomatoNBS-LRRCNLTNLResistance Gene

刘云飞、万红建、韦艳萍、李志邈、叶青静、王荣青、阮美颖、姚祝平、周国治

展开 >

南京农业大学园艺学院,江苏南京 210095

浙江省农业科学院蔬菜研究所,浙江杭州310021

番茄 NBS-LRR CNL TNL 抗病基因

国家“863”计划国家自然科学基金浙江省自然科学基金浙江省农业新品种选育重大科技专项公益性行业(农业)科研专项经费国家科技支撑计划国家大宗蔬菜产业技术体系浙江省蔬菜产业创新团队

2012AA10010031071800,31301774,31272156LQ12C150042012C129032010030652012BAD02B02CARS-25-G-162009R50026

2014

10.11869/j.issn.100-8551.2014.05.0790

核农学报
中国原子能农学会 中国农业科学院农产品加工研究所（前中国农业科学院原子能利用研究所）

核农学报

CSTPCDCSCD北大核心
影响因子：1.5
ISSN：1000-8551
年,卷(期)：2014.28(5)
  • 21
  • 3