Variance Analysis of Streptomyces Venezuelae Snea253 Mutants against Meloidogyne Incognita
The main objective is to evaluate the spontaneous mutants of Streptomyces venezuela Snea253 against Meloidogyne incognita by its metabolites.Six mutates of separate colony morphology were obtained through spontaneous mutation by successively sub-culturing original Streptomyces Snea253 strain,and the morphological differentiation and related genes were analyzed in the paper.Then,the bioactivity of metabolites against Meloidogyne incognita was measured by immersion method in small dish.Six mutants had significant differences in morphology compared with the original strain.Their nematicidal activity were decreased significantly using metabolites treated the secondary juveniles J2 of M.incognita..Among three non-brown pigment producing mutants,the corrected mortality of Snea253-G,Snea253 -B and Snea253-F respectively was 60.0％,45.5％ and 35.0％ to M.incognita J2.Among three pigment producing mutants,Snea253-A,Snea253-H and Snea253-J produced lower nematicidal activity (respectively 48.7％,37.2％ and 37.9％) than that of the mutant Snea253-G(60.0％).It should be noted that there were no difference among the sequences of morphological differentiation related genes (ssgA、bldC、bldD、whiB、whiE、whiI) through sequencing analysis.The six genes may not be directly involved in controlling the spontaneous mutation of Snea253 in here.This research comfirmed that spontaneous mutation of Streptomyces Snea253 strains not only showed in the morphological variation,but also decreased the nematicidal activity.It provides a possibility for screening of biocontrol microbial by morphology.
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NSTL
万方数据
维普
