A Preliminary Research on Improvement of Microspore Embryogenesis for Recalcitrant Broccoli Genotypes
Research on co-culture method of microspores of responsive genotype and recalcitrant genotype to improve efficiency of microspore embryogenesis and obtain more microspore-derived plants of recalcitrant genotype.A responsive genotype of rapeseed (ZS758) and two recalcitrant genotypes of broccoli (Q1 and Q2) were used in isolated microspore co-culture to study microspore embryogenesis process,microspore-derived plants number and type,and plant regeneration.The results showed that the process of microspore embryogenesis of broccoli was same with rapeseed.The no.of embryos per bud,frequency of cotyledon-type embryos and embryo germination of T1-3 (10 buds of ZS758 + 2 buds of Q1) andT2-3 (10 buds of ZS758 + 2budsofQ2) were 12.5 and 14.1,78.3％ and 81.7％,92.6％ and 87.6％,respectively,which were better than control of broccoli.And treatments of T1-2 (6 buds of ZS758 + 6 buds of Q1) and T2-2 (6 buds of ZS758 + 6 buds of Q2) produced 13 and 27 plantlets of Q1 and Q2,which was better than other treatments.Besides,the morphological characters of broccoli and rapeseed were different significantly,which was easier to distinguish.Conclusion,the microspore co-culture method could not only promote the efficiency of embryogenesis to obtain embryoids,but improve the ratio of cotyledonary embryos.The method was important to improve utilization efficiency of microspore culture.
国家科技期刊平台
NSTL
万方数据
维普
