Study on Factors Influence Somatic Embryogenesis From Roots of Lilium spp.
To explore the impact of auxin on somatic embryogenesis ability of Lilium roots,the induction and development of somatic embryos by auxin transport inhibitor (NPA) were studied.Meanwhile,the effect of different root lengths and root segments on somatic embryogenesis and different hormones on somatic embryos amplification were investigated.Results showed that medium supplemented with NPA significantly inhibited the induction of embryogenic callus and produced abnormal somatic embryos.The root explants with root tip could induce embryogenic callus with the callus induction frequency more than 94.4％.The root explants without root tip and the root segments away from root tip induced less embryogenic callus with the callus induction frequency of 8.9％.The root explants with the length of 0.3cm and root tip had the highest growth index (36.8).Embryoids number per root explants (different lengths with root tip) ranged from 21.8 to 26.3 with no significant difference observed.The highest proliferation rate of globular embryos was 98.9％ when globular embryos were cultured on MS medium supplemented with 0.5 mg· L-1 Picloram.The maximum proliferation index was 20.1 when globular embryos were cultured on MS medium supplemented with 0.2 mg· L-1 Picloram.The results indicated that the content of endogenous auxin and exogenous auxin was closely related to somatic embryogenesis of Lilium spp..Endogenous auxin transport was involved in embryonic induction and morphogenesis.The study provided a theoretical basis for the molecular mechanism of Lilium somatic embryogenesis.
国家科技期刊平台
NSTL
万方数据
维普
