The Regulation Effect of the Soybean Auxin Response Factor Gene GmARF17 on the Growth and Development of Hypocotyls
In order to enrich the knowledge of soybean molecular breeding,this paper mainly deals with molecular mechanism of soybean hypocotyls regulated by hormone auxin.Transcripts for GmARF17 were present in cotyledons,hypocotyls and roots in soybean [Glycine max (L.) Merrill],relatively high in lateral roots and lower in hypocotyls using real-time quantitative PCR(qPCR).In hypocotyls,expression of GmARF17 was induced quickly by exogenous auxin IAA,indicating GmARF17 is an immediate-early auxin response gene.Meanwhile,the working model between Gm-miR160 and its target GmARF17 was analyzed by mining soybean genome data.The resistant version vector GmARF17::mGmARF17 (mGmARF17) was constructed and then transformed into wild type soybean.Successful transgenic plants expressing mGmARF17 showed normal leaves,main stems and floral organs but shorter hypocotyls.The decreased concentration of IAA and increased GmGH3.6 were found in the mGmARF17 hypocotyls,implying that GmARF17 regulates hypocotyls cell elongation by modulating IAA homeostasis.However,the response to exogenous auxin in mGmARF17 hypocotyl was found to be similar to that of control.Expression levels of GmSAUR23,GmIAA2,GmARGOS,GmARF10 and GmARF16 are down-regulated but GmMIR160A are up-regulated in the mGmARF17 hypocotyls,suggesting that auxin signaling is suppressed and the balance of between GmMIR160A and its targets is disrupted.Taken together,our paper presents a novel regulatory pathway that the soybean auxin response factor GmARF17 is involved in the growth and development of hypocotyls and likely acts as a negative regulator of cell elongation by regulating chelating gene(s) through the interaction between GmMIR160A and its targets.The results of this paper will benefit soybean molecular breeding.
国家科技期刊平台
NSTL
万方数据
维普
