Clone, Expression and Bioinformation Analysis of Ta-UBX1 Gene in Wheat (Triticum aestivum L.)
In order to study the function of the wheat U-BOX gene,the full-length cDNA of a novel UBX domaincontaining gene,designated as Ta-UBX1,was obtained from wheat varieties Zhoumai 22 and Zhoumai 24 using reverse transcription polymerase chain reaction (RT-PCR) technology.The sequence and expression patterns as well as evolution characteristics of Ta-UBX1 were analyzed.The results showed Ta-UBX1 was 2 058 bp in length,encoded a 553 amino acid protein that contained typical UBA_like and UBX domain in its " N-terminal" and " C-terminal",respectively,which belonged to UBX protein family in ubiquitin regulation pathway,and the amino acid sequence of TaUBX1 protein has higher similarity to the known UBX domain-containing proteins from other gramineous plants,showed high conservative in gramineous plants.Ta-UBX1 gene exhibited significant expression variations in different tissues.Which was more abundant in spikelet flowers and young ear than in other tissues,and showed higher expression amount from early tetrad stage to mononuclear stage,then gradually reduced from late-mononuclear to trinucleate stage during anthers development,which suggested it might be involed in floral development,especially in male gametophyte development in wheat (Triticum aestivum L.).These results provide a molecular basis to understand the function of TaUBX1 gene.
国家科技期刊平台
NSTL
万方数据
维普
