Study on EST-derived SSR Marker Development of Chrysanthemum morifolium and Its Transferability to Tagetes erecta L.
This study aims to characterize EST-SSR in Chrysanthemum morifolium, development SSR markers and clarify the transferability of them to Tagetes erecta.With these sequences downloaded from NCBI, SSR sites occurred with an occurrence frequency of 5.35％ (1 per 10.64 Kb).Among the 83 kinds of repeat motifs, di-, tri-, te-, pe-,and he-nucleotides were 10, 40, 14, 5 and 14 types, respectively.Using 14 cultivars of Chrysanthemum morifolium as templates, 14 among 22 pairs of primers were effective.In this process, 49 polymorphism sites were detected with average values of 3.5 bands per primer pair and 0.751 of PIC.Furthermore, among 14 pairs of primers, clear and stable bands could be amplified by 8 primer pairs using Tagetes eracta as templates, with a transferability rate of 57.14％.Taken together, trinucleotide type was dominant in Chrysanthemum morifolium, and henucloetide types were more than these in other species.At the sametime, these EST-SSRs contained higher PIC values.These primers from Chrysanthemun morifolium EST could be used as molecular markers of Tagetes eracta.
国家科技期刊平台
NSTL
万方数据
维普
