首页|花生GLP家族基因组织差异及干旱诱导的表达分析

花生GLP家族基因组织差异及干旱诱导的表达分析

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为深入探索花生GLP家族基因的功能,利用实时荧光定量PCR技术比较不同花生品种种皮和干旱胁迫下花生不同组织中花生GLP家族基因的表达情况.结果表明,花生GLP家族基因在粉红色花生品种种皮中的表达量显著高于其它颜色种皮;在花生种皮中,AhGLP3和AhGLP6的表达量相对最高,其次是AhGLP1、AhGLP2和AhGLP8.正常生长条件下,除AhGLP2和AhGLP8,其它各基因在种子、根和叶中的表达均有明显的组织差异.在干旱胁迫下,AhGLP1在叶片中的表达量发生上调,而AhGLP3、AhGLP4和AhGLP5在种子和根中的表达量显著上调;AhGLP2、AhGLP7和AhGLP8在各组织中均受干旱诱导而表达量上调,而AhGLP6的表达量下降.结果表明花生GLP家族基因的表达有明显的品种特异性、组织和诱导差异性.本研究为阐明花生GLP家族基因的组织及抗逆表达提供理论基础.
Analysis of Peanut GLP Family Genes: Tissue-specific Expression and Induction in Response to Drought Stress
In order to study the function of the peanut GLP family genes,differential expression of eight AhGLPs between peanut testa,seed,leaf and root was analyzed under drought condition.Results showed that expression of the eight AhGLPs were higher significantly in pink testa than in others.Moreover,in peanut testa,AhGLP3 and AhGLP6 expressed highest,and followed by AhGLP1,AhGLP2 and AhGLP8.Under drought stress condition,AhGLP1 was induce-expressed only in leaves.AhGLP3,AhGLP4 and AhGLP5 were up-regulated significantly in seeds and root,and the expression of AhGLP2,AhGLP7 and AhGLP8 were induced in root,seeds and leaves by drought stress.On the contrary,AhGLP6 was down-regulated by drought stress.Under the condition of normal growth,AhGLP1,AhGLP3,AhGLP4,AhGLP5,AhGLP6 and AhGLP7 were expressed with tissue-specificity.The above results indicate that the expression of peanut GLP family genes have species-and tissue-specificity under different conditions.It could provide valuable information for the fuctional and regulatory stydies on peanut AhGLPs genes especially under drought stress.

peanut (Arachis hypogaea.L)GLP family genestissue expressiondrougth stress

王通、梁炫强、王冕、陈小平、潘丽娟、陈明娜、陈娜、迟晓元、禹山林

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山东省花生研究所/农业部花生生物学与遗传育种重点实验室,山东青岛266100

广东省农业科学院作物研究所,广东广州510640

中国农业科学院油料作物研究所/农业部油料作物生物学与遗传育种重点实验室,湖北武汉430062

花生 GLP家族基因 组织表达 干旱

国家花生产业技术体系项目国家自然科学基金农业部油料作物生物学与遗传育种重点实验室开放课题基金

CARS-14312002112014010

2016

核农学报
中国原子能农学会 中国农业科学院农产品加工研究所(前中国农业科学院原子能利用研究所)

核农学报

CSTPCDCSCD北大核心
影响因子:1.5
ISSN:1000-8551
年,卷(期):2016.30(3)
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