Construction of Fingerprinting and Analysis of Genetic Diversity With SSR Markers for Forty-Six Approved Peanut Cultivars From Shandong Province
In this study,46 peanut cultivars of Shandong province were used as materials in construction of DNA fingerprinting and analysis of genetic diversity with simple sequence repeat (SSR) markers.50 of 788 pairs of SSR primers with high polymerphism,stability and clear bands were screened out.The 50 primer pairs amplified a total of 175 alleles (including 122 polymorphic alleles) among the 46 cultivars,and the ratio of polymorphism was as high as 70.52％.Alleles amplified by each pair of primers ranged from 2 to7.The polymorphic information content values (PIC) ranged from0.6753 to 0.8412,with a mean of 0.823.These 46 peanut cultivars could be absolutely identified with only 14 primer pairs.UPGMA cluster analysis of genetic similarity showed that all the materials were clustered in to one group at the genetic similarity of 0.77,and 76％ of the cultivars were still clustered together at the genetic similarity of 0.80.The genetic relationships of cultivars were identical to the family tree basically.It is indicated that the genetic basis of peanut cultivars in Shandong province is narrow.Construction of fingerprinting with SSR markers will provide the basis for peanut germplasm resources management and breeding.
国家科技期刊平台
NSTL
万方数据
维普
