Effect of Pretreatment and Hormone on Anther Culture of Strawberry Fragaria ananassa Var.Hongjia in Vitro
Anther culture is an effective method for plant detoxification and to get haploid.In order to raise the regeneration efficiency of anther culture of strawberry,pretreatment of strawberry (Fragaria ananassa var.Hongjia) anther in low temperature and dark condition,calli and adventitious shoots induction of different auxine from anthers were studied as well.The results show that low temperature treatment under 4 ℃ condition for 72 h before culture can promote the formation of callus,whereas,it was no useful for induction of callus with treating anthers in darkness.High concentrations of 2,4-D (MS + 1.0mg· L-1 6-BA + 2.0mg· L-12,4-D) contributed to inducing callus,special for valid ones.NAA played a more important role than other auxins in adventitious shoots induction,especially MS + 1.0 mg·L-1 6-BA +0.2 mg· L-1 NAA had a high effiency and more than 10％ calli can be induced to produce shoots.Cytokinin and auxin should not be added with too high concentration in adventitious shoot proliferation process,otherwise,shoots cannot develop to healthy plants,and the medium of MS containing 0.7mg· L-16-BA and 0.1 mg· L-1 NAA was the best.Virus free plant and haploid of strawberry can be obtained via anther regeneration in high frequency by theway.
国家科技期刊平台
NSTL
万方数据
维普
