Cloning of a Dehydrin Gene PcDHN1 and Its Response to Abiotic Stresses in Sweet Cherry Rootstock
Abiotic stresses are major factors which significantly affecting the growth and yield of fruits.To explicit the characteristics of a dehydrin gene and its response to drought,high-salt and cold,RT-PCR and RACE technologies were used for cloning of PcDHN1 and bioinformatic methods were applied for characterizing the dehydrin gene and its deduced protein.The gene expression patterns were analyzed by using the real time qRT-PCR.A sweet cherry rootstock dehydrin gene PcDHN1 was cloned and sequencing analysis showed that the cDNA of the dehydrin gene is 893 bp in length,and encodes 225 amino acids with a predicted molecular weight of about 23.98kD and pI of 8.65.Amino acid sequence analysis indicated that PcDHN1 possessed typical characters of LEA family and was sorted into Yn SK2 subfamily of dehydrins.The expression analysis revealed that PcDHN1 was induced by drought-,salt-and chilling-stresses,while insensitive to the chilling-stress.It was deduced that PcDHN1 might play important roles in stress tolerance of drought and high-salt.These results provided references for stress-resistant genetic engineering breeding of sweet cherry rootstocks.
国家科技期刊平台
NSTL
万方数据
维普
