To understand the effects of HMW-GS on quality properties in spring wheat and correlation of different score system of HMW-GS,the relationship between HMW-GS composition or number and quality properties in spring wheat was studied,the different score system of HMW-GS were evaluated.The results show that the wheat quality properties in spring wheat which have Null subunit are worse than those have 2 * subunit or 1 subunit.Glu-B1 loci have little effect on quality properties of spring wheat.17 + 18 subunit was just better than 7 + 8 subunit in the gluten index and than 7 + 9 subunit in extensibility(L).There was obvious effect of the different HMW-GS composition on wheat properties in GluD1 loci.5 + 10 subunits were better than 2 + 12 subunits;there were difference or significant difference in gluten index,granularity,ash,P,W,IE,PRMAX and WA.Based on analysis,the contribution in Glu-1 loci to the sping wheat quality properties was Glu-D1 > Glu-A1 > Glu-B1 in Glu-1.The Payne's score system was the most correlative in four score systems of HMW-GS.The results could provide useful reference for early generation selection in spring wheat breeding.