In order to explore the effect of nitrogen rate and irrigation after anthesis on photosynthesis and filling characteristics in black wheat,a pot experiment with two-factor randomized block design was carried out at the experimental station of the Institute of Crop Science of Chinese Academy of Agricultural Sciences in 2014-2015 to study the effect of nitrogen rate and soil relative water content after anthesis on photosynthesis and filling characteristics in black wheat.The tested black wheat varieties was Luozhen No.1.There were three nitrogen application rates at 150 kg (N) ·hm-2,240 kg (N) ·hm-2,and 330 kg (N)·hm-2,respectively,which were defined low,middle,and high treatments,and marked as N1,N2,N3.Three levels of soil relative water content were applied at 75% to 85% (Adequate water supply treatment),55% ~ 65% (middle water stress treatment) and 35% to 45% (serious water stress treatment),and marked as W1,W2 and W3,respectively.The result shows that SPAD and Pn are increased with adequate water supply treatment of 75% ~ 85%,but declined with serious water stress treatment of 35% to 45%.Theoretical highest kernel weight,max filling rate,average filling rate and filling duration were the highest for W1 treatment and the lowest for W3 treatment.Max filling rate and average filling rate were increased with increasing of nitrogen application rate at the serious water stress treatment.The most suitable nitrogen application amount and water supply were 240kg·hm-2 and adequate water supply treatment.This study provides a theoretical basis for higher yield of black wheat.