Study on the Extraction Technology of Walnut Oil by Aqueous Enzymatic Method
In order to optimize the aqueous enzymatic extraction of walnut (Caryacathayensis Sarg.) oil.The extraction process of walnut oil was studied by using aqueous enzymatic method.The single factor experiments and response surface were conducted for analysis of papain concentration,enzymatic time,pH value,enzymatic hydrdysis temperature and liquid-to-solid ratio on the extraction rate of walnut oil.The fatty acid compositions of the walnut oil were analyzed by GC-MS and the physicochemical properties of walnut oil extracted with pressing method,solvent extracting and aqueous enzymatic methods were determined and compared.Results showed that the optimum technological condition for walnut:enzyme concentration was 0.17％,enzyme reaction time was 150 min,pH value 6.34,extraction temperature was 54.43℃Cand liquid-to-solid ratio was 1:5 by analysis of response surface method.Under this condition the extraction ratio of walnut oil was 81.32％.Twelve fatty acids were found in walnut oil by GC-MS,The palmitic acid,oleicacid,inoleic acid and linoleic acid were the four main ingredients,the content of saturated fatty acid(SFA),monounsaturated fatty acid (MUFA) and polyunsaturated fatty acid (PUFA) were 7.61％,69.10％ and 23.29％,respectively.Comparison of three methods for oil extraction,aqueous enzymatic method was the ideal extraction of oil.This research provided theoretical basis for oil extraction by using aqueous enzymatic method.
国家科技期刊平台
NSTL
万方数据
维普
