Cloning of Gene PmNCED1 and Its Response to PEG Stress in Common Millet
To explicit the characteristics of 9-cis-epoxycarotenoid dioxygenase (NCED) gene and its response to drought stress,a NCED from common millet Huangmizi,named PmNCED1,was cloned and the characters of PmNCED1 protein were predicted.Results were shown as follows.PmNCED1 contains a 1284 bp open reading frame,encodes a 428-amino acid protein.The predicted protein belongs to carotenoid cleavage dioxygenases family and NCED subfamily domain,contains one amphipathic α-helix region of AA56-75 (-YARAACGLVDPSHGTGIANA-) and four conserved histidine residues.AA23-29 (-ARLRQER-) and AA284-290 (-TGRSTRR-) were predicted as nuclear localization signal,AA400 was predicted as nuclear export signal.The deduced amino acid sequence of PmNCED1 protein shared 77％-96％ sequence identity with that of other plants.PmNCED1 protein constituted of 4 α -helixs and 25 extended strands.Quantitative Real-time PCR result showed that expression of PmNCED1 increased with PEG stress time increasing.Under PEG-6000 stress,changes of physiological indicators together with volume of bulliform cell showed significant differences (P ＜ 0.01).It suggests that PmNCED1 was related to PEG stress.This study provide a theoretical basis for enhancing the drought tolerance of other crops such as wheat,etc.
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万方数据
维普
