Study on the Inhibitory Effect of Bacillus velezensis on Fusarium graminearum
To enrich the biological control strain resources of wheat scab,a strain JS25R was obtained by antagonistic trials against Fusarium graminearum from the infected wheat spikelets in this study.The strain JS25R exhibited effective antifungal activity against F.graminearum and also efficiently reduced disease severity,disease incidence and disease index.Based on biochemical and physiological characteristics,and 16S rDNA analysis,the selected strains was identified as Bacillus velezensis.The inhibition mechanism of this strain against F.graminearum was further investigated by antagonistic effect of cell free culture and volatile compounds (VOCS) produced by JS25R.It showed that cell free culture of JS25R could effectively reduce the growth of F.graminearum with an inhibition zone of 0.58 cm.The presence of five antimicrobial peptide genes were checked by PCR.Amplification of these gene markers showed that each gene had one specific band with the right size.Besides,VOCS produced by JS25R could reduce the growth of F.graminearum by 27.7％ and the germination by 26.7％.Among these VOCS,disulfide dimethyl,2-undecanone,2-nonanone and 2-nonanol could completely inhibit the growth of F.graminearum.In total,36 volatile compounds of JS25R were detected by GC-MS,mainly ketones,alkanes,benzenes,alcohols and so on.The results from this research suggested that Bacillus strains could be a potential candidate antagonist of fusarium head blight.
Bacillus velezensisFusarium graminearumfusarium head blightbiocontrol agent
国家科技期刊平台
NSTL
万方数据
维普
