Study on the Biological Effects of High-Energy Heavy Ion Beam Irradiation on Astragalus mongholicus Seedlings
This study aims to investigate the biological effects of different doses of high-energy heavy ion beam(HIB)irradiation on the seedlings of Astragalus mongholicus(A.mongholicus),a traditional Chinese medicinal plant,and to determine the optimal dose for HIB irradiation mutagenesis breeding.The 7-day-old seedlings of A.membranaceus were exposed to different doses of HIB irradiation,and the survival rate,seedling height,leaf number,stem diameter,fresh root weight,and leaf morphology parameters were measured on the 10th day after irradiation.Chlorophyll content,chlorophyll fluorescence parameters,antioxidant enzyme activities,and oxidative indices were determined in the leaves at 20-day intervals,starting 20 days after irradiation.The results showed that with increasing irradiation dose,the survival rate,plant height,and fresh root weight of seedlings decreased compare to the control group.After irradiation with 5 Gy and 10 Gy,the fresh root weight decreased by 31.80%and 49.99%,respectively.The leaves on the first and second branches of the irradiated groups exhibited deformities,shriveling,and morphological damage.On the 20th day after irradiation,the levels of reactive oxygen species,membrane lipid peroxides,and chlorophyll content in the irradiated groups decreased significantly compared to the control group.However,by the 60th day,these levels were comparable to those in the control group.After irradiation,there was a slight increase in Fv/Fm,while the chlorophyll fluorescence parameters of the seedlings decreased.These results suggest that as the dose of HIB irradiation dose increased,the growth and development of A.membranaceus seedlings were inhibited.However,the inhibitory effect on seedling growth was alleviated as the plant grew post-irradiation.The recommended dose range for optimal results is approximately 10 Gy.This study provides essential basic data for HIB irradiation mutagenesis breeding of A.membranaceus.
Astragalus mongholicusheavy ion beamirradiationphotosynthetic characterbiological effects
万方数据
维普
