Mutagenesis Technique Breed Pleurotus Citrinopileatus High Yield of Ergothioneine
Ergothioneine is a naturally accuring active substance found in edible fungi,known for its antioxidant and metal chelating functions.In order to further explore and improve the yield of ergothioneine produced through biological fermentation,this study used ultraviolet mutagenesis(UV),ethyl methylsulfonate mutagenesis(EMS)and space mutagenesis(SFM)to mutate strains of the Pleurotus Citrinopileatus.The mycelial growth rate and liquid fermentation yield of ergothioneine were used as evaluation indicators to select high-yield straits of Pleurotus Citrinopileatus.The results showed that 46,40 and 48 mutant strains were obtained through UV,EMS and SFM mutagenesis,respectively.The positive mutation rate for UV mutagenesis was 43.48％.UV,EMS and SFM mutagenesis resulted in 8,3 and 1 strain of liquid-fermented ergothioneine,respectively,with significantly higher yields than the original strain(P＜0.01).Among the 12 high-yielding ergothioneine mutagenic strains,the yield of the UV-30 strain was the highest,at 2.16 times that of the original strain,while the growth rate of the UV-13 strain was the fastest,at 1.02 times that of the original strain.Genetic distance analysis showed that the mutation degree of the SFM mutant strain was higher than that of the UV mutant strain,and both strains exhibited a higher mutation degree of both strains the EMS mutant strain.The results of this study contribute valuable material for the fundamental research of the ergothioneine biosynthesis pathway and provide a reference for the mutagenesis and breeding of the high yield ergothioneine strain of Pleurotus Citrinopileatus.
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维普
