Evaluation of Different Tartary Buckwheat Germplasm Resources for Shade Tolerance and Screening of Identification Indexes
In order to screen Tartary buckwheat germplasm resources for shade tolerance and establish a model for evaluating this trait,60 Tartary buckwheat germplasm resources were used as materials.Field experiments were conducted under two treatments:shaded and natural light.Nine morphological structure indicators such as plant height,and six physiological indicators such as superoxide dismutase(SOD)activity,were measured.The shade tolerance coefficient for each indicator was calculated.Multivariate statistical analysis methods were used to comprehensively assess the shade tolerance of Tartary buckwheat and to identify key indicators of shade tolerance.The results indicated varying degrees of variation in the shade tolerance coefficients among the 15 indicators,with variation coefficients ranging from 7.87％to 85.84％.With the exception of malondialdehyde(MDA)content,peroxidase(POD)activity,and catalase(CAT)activity,a certain correlation was observed among the other individual indicators.Principal component analysis was used to extract five principal components from the shade tolerance coefficients of the 15 indicators,resulting in a cumulative variance contribution rate of 69.31％.The comprehensive evaluation value(D)for the 60 Tartary buckwheat germplasm resources was calculated using the membership function analysis method.Subsequently,cluster analysis categorized the germplasm resources into three categories:shade-tolerant,moderately shade-tolerant,and shade-sensitive.Further,stepwise regression analysis was used to establish the optimal linear regression equation:D=-0.176+0.115X1+0.313X5+0.140X6+0.057X7+0.176X10-0.015X13.Six indicators such as plant height,fifth internode thickness of the main stem,eighth internode thickness of the main stem,number of seeds per plant,relative chlorophyll content at the beginning of flowering(SPAD),and POD activity were selected as indicators for assessiing shade tolerance.The results of this study provide a reference for the identification of Tartary buckwheat germplasm resources with shade tolerance and for the breeding of new varieties.
万方数据
维普
