Molecular Mapping of the Frl Gene for Resistance to Fusarium Crown and Root Rot in Tomato
Fusarium crown and root rot(FCRR)of tomato(Solanum esculentum)is one of the most damaging soil-borne diseases affecting tomatoes in recent year,significantly impacting both the quality and yield of tomato.To explore candidate genes associated with resistance to FCRR in tomatoes,a genome-wide association study(GWAS)combined with genetic mapping was used.A total of 200 tomato materials from around the world were used for the GWAS,leading to the identification of a major locus associated with the resistance to FCRR on tomato chromosome 9.A genetic linkage map was constructed using a parental population derived from a cross between the resistant line KP13 and the susceptible line T15196.Based on the results of the GWAS and linkage mapping,the resistance locus(Frl)was mapped between the markers FrlSNP20 and SSR105,with a genetic distance of approximately 0.8 cM and a physical distance of about 93 kb.Four candidate genes belonging to the tomato glutathione S-transferase(S1GST)gene family were identified,including Solyc09g011510.2.1,Solyc09g011580.2.1,Solyc09g011600.2.1 and Solyc09g011630.2.1.The FrlSNP25 marker,which is tightly linked to the resistance of FCRR,was obtained and used to identify the resistance of 94 tomato accessions.The concordance rate between genotypic and phenotypic resistance was 92.55％for Fusarium crown and root rot.This study lays a foundation for the innovation of tomato germplasm and the understanding of resistance mechanisms in tomato against Fusarium crown and root rot.
tomatoFusarium crown and root rotgene mappingresistance identification
万方数据
维普
