CAPS Marker of Cytoplasmic Male Sterility Restorer-of-fertility Gene in Pepper(Capsicum annuum)Converted to PARMS Marker and Its Application
Co1Mod1-CAPS is a molecular marker that could be widely used to screen for sources carrying restorer-of-fertility gene associated with cytoplasmic male sterility in pepper.In order to enhance its utility as a high-throughput detectable molecular marker and improve selection efficiency,10 three-line(sterile line,maintainer line and restorer line)materials belonging to different types of pepper were amplified using Co1Mod1-CAPS marker,and the sequence differences of the amplification products amongthem were analyzed.Based on these sequence differences,we converted Co1Mod1-CAPS marker into PARMS marker,which allows for high throughput detection.The PARMS marker was subsequently employed to screen 321 for materials carrying the restorer-of-fertility gene across 321 pepper germplasm sources.The results revealed a T→C base variation at the 601st position of the Co1Mod1-CAPS marker amplification product,with T in sterile and maintainer lines and C found in restorer lines.Based on this base variation,we successfully converted Co1Mod1-CAPS into the high-throughput marker Co1Mod1-PARMS,and screened out 146 resources carrying the homozygous restorer-of-fertility gene RfRf among the 321 germplasm materials.Among them,the small-fruit-shaped and spicy pepper had a higher proportion of individuals carrying the RfRf genotype.The short finger-shaped pepper had the highest proportion at 59.5％,followed by cherry pepper at 56.5％,while the sweet pepper(lantern pepper)had the lowest proportion at 8.3％.The results of this study are consistent with previous research,confirming the effectiveness of Co1Mod1-PARMS.Therefore,this high-throughput marker can be used for molecular marker-assisted breeding of cytoplasmic male sterile restorer lines in peppers,thereby improving the breeding efficiency of cytoplasmic male sterile restorer lines.
peppercytoplasmic male sterilerestorer-of-fertility genehigh-throughputmolecular marker assisted breeding
万方数据
维普
