Determination of Safe Dose and Quality Evaluation for Sterilization of Salvia miltiorrhiza by Electron Beam Irradiation
To study the effect of electron beam irradiation on the quality of Salvia miltiorrhiza,this study treated Salvia miltiorrhiza samples with irradiation doses of 2,4,6,8,10,12,14,16 and 18 kGy,respectively.and compared the microbial level,8 compounds content and antioxidant activity of Salvia miltiorrhiza at different doses.The optimal safe dose was determined by orthogonal partial least square discriminant analysis model.The fingerprints of Salvia miltiorrhiza before and after irradiation were established to determine the chemical composition consistency.Six batches of salvia miltiorrhiza were selected to verify the optimal safe irradiation dose.The results showed that the microbial level was in line with the pharmacopoeia standard of the People's Republic of China Pharmacopoeia 2020 Edition No.4 when the irradiation dose of Salvia miltiorrhiza electron beam sterilization reached 10 kGy or above,.The total content of 8 compounds of salviorrhiza was within 10 kGy,and there was no significant difference between the irradiation group and the control group.There were no significant differences in the antioxidant activities of the 4 kinds at the dose of 10 kGy,and the antioxidant activities of the 10 kGy dose group were the closest to the control group.In conclusion,10 kGy was the best safe irradiation dose for Salvia miltiorrhiza electron beam sterilization.The similarity of fingerprints of Salvia miltiorrhiza by high performance liquid chromatography(HPLC)and Raman spectroscopy(SERS)before and after irradiation was greater than 0.9 indicating good consistency of chemical components.After 6 batches of Salvia miltiorrhiza were sterilized by 10 kGy,the microbial levels were in line with pharmacopoeia regulations.There were no significant differences in the contents of 8 compounds,antioxidant activity and survival rate of hepatic stellate cells(HSC-T6)by t test.The results of this study can provide scientific basis for the application of electron beam irradiation sterilization technology in the production and quality evaluation of Salvia miltiorrhiza.
Salvia miltiorrhizaelectron beam irradiation sterilizationantioxidant activityfingerprintsquality evaluation
万方数据
维普
