Effect of N-acetylcysteine on Reactive Oxygen Metabolism and Glycolysis of Lentinula edodes Mycelia under Heat Stress
In order to explore the effect of exogenous N-acetylcysteine(NAC)on the active oxygen metabolism and glycolysis of the Lentinula edodes mycelia under heat stress,this study took the strain with weak heat resistance as the test strain,and set different concentrations of exogenous NAC into the medium.The optimal addition concentration was selected under 37℃.With this concentration,the mycelia was subjected to heat stress(0,6,12,24 h)under 37 ℃,and the growth recovery after heat stress(6 h after 24 h of heat stress at 25 ℃),and the related indexes of active oxygen metabolism and glycolysis were determined.The results showed that 10-1 mmol·L-1 was the best additive concentration.During heat stress,the peak contents of superoxide anion,hydrogen peroxide,protein carbonyl and thiobarbituric acid reactants in NAC treatment were significantly decreased by 40.94％,41.97％,53.21％and 47.62％,respectively,compared with that without NAC(control group).The activity of superoxide dismutase,catalase,peroxidase,ascorbate peroxidase,hexokinase and pyruvate kinase and the content of reduced glutathione were significantly increased by 19.21％,43.98％,12.36％,21.95％,25.42％,54.41％and 31.93％,respectively.At the same time,the contents of active oxygen substances and oxidative damage products in the mycelia treated with NAC were still significant lower than those in the control group,while the activities of superoxide dismutase,peroxidase,ascorbate peroxidase,hexokinase and pyruvate kinase and the contents of reduced glutathione were still significant higher than those in the control group.NAC effectively maintained the homeostasis of active oxygen species in mycelia during heat stress,kept the activity of antioxidant enzymes and reduced glutathione at a high level,slowed down the degree and process of oxidative damage,and accelerated the glycolysis pathway of mycelia.Multivariate analysis of variance showed that NAC had significant effects on glutathione,thiobarbituric acid reactants,superoxide anion content and the activities of hexokinase,catalase and ascorbate peroxidase.The results of this study provide a new idea for the application and promotion of exogenous NAC in edible fungi during heat stress.
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