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海南医科大学学报（英文版）
海南医科大学学报（英文版）

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海南医科大学学报（英文版）/Journal Journal of Hainan Medical University
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收录年代

    Screening and functional analysis of the long-range interaction elements of β-globin genes

    XIAO Yi-shuXU LanLIU Chun-yaDU Le...
    1-7页
    查看更多>>摘要：Objective: Studies have shown that β-globin gene presents a selective expression transformation mechanism during development, and its upstream locus control region (LCR) regulates the expression pattern of β-globin gene family. To further explore the molecular network of β-globin gene expression regulation, other long-range regulatory elements that may be involved in the regulation of β-globin gene expression were screened and the dynamic regulation and transformation mechanism of β-globin gene was deeply studied. Methods: Promyelocytic cells were induced to differentiate by all-trans retinoic acid. β-globin gene promoter region and LCR were used as the target sites for circular chromosome conformational capture (4C) analysis. Through sequencing and regulatory element analysis, the sites interacting with β-globin family loci were screened in the whole genome. Results: According to the results of 4C sequencing, the sites that interact with HBD promoter region and LCR were screened. Verified by chromosome conformational capture (3C), the results were consistent with those of sequencing. The functional analysis of regulatory elements by formaldehyde-assisted separation regulatory elements and Epiregio online website showed that the screening sites AC105129.4, AL354707.17, AC078785.22 and AC021646.35 were all potential regulatory elements involved in β-globin gene. Conclusion: The interaction between 4C screening site and anchor site showed the complex spatial organization of β-globin family loci in the nucleus.
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    Expression and correlation of pyruvate kinase M2 and miRNA-122 in sepsis associated AKI

    FU ZhouLI TingSONG XuLI Ru-xin...
    8-13页
    查看更多>>摘要：Objective: To investigate the expression and correlation of pyruvate kinase M2 and miRNA-122 in sepsis associated acute kidney injury (S-AKI). Methods: A mouse model of S-AKI was induced by cecal ligation and perforation (CLP), and normal mice were used in the control group. Serum and renal tissue were collected from each group, respectively, and the levels of blood urea nitrogen (BUN) and creatinine (Cr) were detected by biochemical analyzer. The levels of lactate in serum and kidney tissue of mice in each group were detected by colorimetric method. Real-time quantitative PCR (RT-qPCR) was used to detect the expression of miRNA-122 and pkm2 mRNA in kidney tissue in each group. Western blotting was used to detect the expression of PKM2 protein in kidney tissue in each group. Pearson's method was used to analyze the pairwise correlation of miRNA-122, PKM2 and lactate levels. Results: (1) Compared with the control group, the levels of BUN and Cr increased significantly after 12 h and 24 h of CLP treatment (P <0.05). (2) Compared with the control group, after 12 h and 24 h of CLP treatment, the levels of lactate in the serum and kidney tissue of the mice were significantly higher than those in the control group (P<0.05). (3) Compared with the control group, the expression of miRNA-122 in renal tissue began to decrease at 4 h after CLP, and decreased significantly at 12-24 h (P<0.05). Compared with the control group, the expression of pKm2 mRNA and protein in renal tissue began to increase after 4 h of CLP, and increased significantly at 12-24 h (P<0.05). (4) Correlation analysis showed that miRNA-122 was significantly negatively correlated with lactate level (P<0.0001, r=-0.7167). There was a significant positive correlation between pkm2 mRNA and lactate level (P<0.0001, r=0.6817). There was a significant negative correlation between miRNA-122 and pkm2 mRNA expression (P<0.0001, r=-0.8122). Conclusion: In S-AKI, dysregulated expression of miRNA-122 may aggravate the occurrence and development of AKI by regulating the level of PKM2, and promoting aerobic glycolysis and lactate levels.
      原文链接:
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    Effect of lentiviral expression of circLIFR on the biological behavior of Hep3B hepatocellular carcinoma cells

    LI ChenLIU Lu-zhengLU Han-yuanCHEN Jia-cheng...
    14-18页
    查看更多>>摘要：Objective: To investigate the effect of lentiviral stable high expression of circLIFR on the biological behavior of Hep3B hepatocellular carcinoma (HCC) cells. Methods: Hep3B cell lines were infected with lentiviral packaging of circLIFR expression plasmids to construct stable circLIFR high expression HCC cells. The lentivirus infected with circLIFR high expression sequence was used as the circLIFR high expression group, the lentivirus infected with circLIFR high expression empty vector sequence was used as the negative control group, and the uninfected group was used as the blank control group. After that, circLIFR expression levels were detected by qPCR, and the back splice sites were identified by Sanger sequencing. The cell viability was examined by cell proliferation kit and invasive ability was determined by Transwell assay. Results: The qPCR and Sanger sequencing showed that the stable circLIFR expression of Hep3B cells was successfully established. The circLIFR high expression group had better cell proliferation viability than the negative control and blank control groups, and the differences were statistically significant (P <0.05). The number of cells crossing Matrigel gel in the negative control and blank control groups was (270.8±18.9) and (266.2±17.6), respectively, while the number of cells crossing Matrigel gel in the circLIFR high expression group was (396.6±32.9), and the differences were statistically significant (P<0.05). Conclusion: High circLIFR expression considerably promotes the proliferation and invasive ability of Hep3B cells.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Effect of Drynaria total flavonoids on the expression of NMDAR1, GluR2 and CaMK Ⅱ in the brain of hydrocortisone model mice

    WANG Yi-tongXU Yan-mingZHANG Zhi-boSUN Hui-feng...
    19-24页
    查看更多>>摘要：Objective: To study the effect of total flavonoids of Rhizoma Drynariae on the expression of NMDAR1, GluR2 and CAMKⅡ protein in model mice of kidney deficiency induced by exogenous glucocorticoid hydrocortisone and its mechanism.Methods: Kunming (KM) mice were randomly divided into the blank group, the hydrocortisone model group, the anti-brain failure capsule group, the Drynaria total flavonoids group, the Drynaria total flavonoids+ER blocker group, with 15 animals in each group. Except for the blank group, all groups were injected intramuscularly with hydrocortisone (25 mg·kg-1·d-1) to create models. The water maze experiment, new object recognition experiment and platform jump experiment were used to conduct behavioral investigations. HE staining was used to observe the pathological changes of mouse hippocampus, and Western blotting detected the expressions of NMDR1, GluR2 and CAMKⅡ proteins in the hippocampus of mice in each group. Results: The experimental results showed that compared with the model group, the learning and memory ability of the mice in the Drynaria fortunei total flavonoids group was significantly improved, and the difference was statistically significant (P <0.01), the expression of NMDR1 and GluR2 proteins in the hippocampus of the mice was significantly increased (P<0.01), and the level of CAMKⅡ protein significantly decreased (P<0.01). Conclusion: The total flavonoids of Rhizoma Drynariae may enhance the expression of NMDAR1 and GluR2 protein in the brain of hydrocortisone model mice through ER, reduce the expression of CAMKⅡ protein, and alleviate the damage to the brain tissue of the model mice and play a neuroprotective effect.
      原文链接:
    • NETL
    • NSTL
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    Investigation of paeonol-geniposide on acute alcoholic liver injury based on uniform design method

    LIU KeLIU Yu-longSUN MinLIU Ling-ling...
    25-31页
    查看更多>>摘要：Objective: To explore the optimal ratio and compatibility effect of paeonol-geniposide combination on acute alcoholic liver injury by uniform design. Methods: Lieber-DeCarli alcoholic liquid feed was used to induce acute alcoholic liver injury in mice. Uniform design was used to select the best dosage combination of paeonol and geniposide, and the related indexes of liver injury and oxidative stress were detected by kit. Serum inflammatory factors were detected by ELISA, and the expressions of p38 MAPK, JNK and NF-κB P65 related proteins in liver were detected by Western-blot. Results: The regression equation suggested that paeonol: geniposide = 220: 20 was the best ratio of paeonol and geniposide to resist alcoholic liver injury. Compared with the model group, the liver injury indexes and oxidation products of the paeonol+geniposide group decreased significantly, the antioxidant activity of liver tissue increased significantly, and the expression levels of p-p38 MAPK, p-JNK and NF-κB P65 protein decreased significantly. Conclusion: The optimal dosage of paeonol-geniposide was effectively optimized by uniform design and pharmacodynamic analysis. The combination of the two drugs could reduce the alcoholic liver injury by reducing the oxidative stress injury and inflammatory response in the liver tissue of mice, and its effect might be related to the targeting of p38 MAPK/JNK/NF-κB channel.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    A nomogram model for predicting the prognosis of colorectal cancer patients based on preoperative Fbg, PAR and CA199

    LIU Xiu-lingYU Hua-yiQI Guo-pingLU Wen-bin...
    32-38页
    查看更多>>摘要：Objective: To construct a nomogram model for predicting the prognosis of colorectal cancer patients based on preoperative fibrinogen (Fbg), platelet to albumin ratio (PAR) and carbohydrate antigen 199 (CA199). Methods: In this retrospective study, we included 323 patients who underwent colorectal cancer (CRC) surgery in our hospital. The preoperative test indexes and relevant clinicopathological data of the patients were collected. According to the cut-off value of Fbg and PAR calculated from the receiver operating characteristic (ROC) curve, they were divided into the high and low expression groups. Univariate and multivariate Cox proportional hazards models were used to screen independent prognostic factors which were used to construct a nomogram. Results: According to ROC curve, the cut-off values of Fbg and PAR were 2.80 and 6.05. Multivariate Cox regression analysis showed that Fbg, PAR, CA199, TNM stage and grade were independent risk factors affecting the prognosis of patients (P <0.05). Fbg, PAR, and CA199 jointly built the risk score that was included into the nomogram. The area under the curve (AUC) in the ROC of the training and verification set was greater than 0.6. The calibration curve and ideal curve fit well. Conclusion: The nomogram based on Fbg, PAR and CA199 has well accuracy and can provide individualized prediction for the overall survival of CRC patients.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Analysis of key pathogenic target genes of ovarian cancer and experimental verification of cells in vitro

    WANG Yi-hanCHEN Bo-cenPENG Yun-hua
    39-46页
    查看更多>>摘要：Objective: To mine genes highly related to the pathogenesis of ovarian cancer by using multi-chip integrated bioinformatics methods and verify them in cells, which provided key genes and important theoretical basis for targeted research of ovarian cancer. Methods: Three datasets, GSE38666, GSE40595 and GSE54388, were downloaded from the Gene Expression Integrated Database database for differential gene (DEGs) screening, including 26 normal samples and 65 ovarian cancer samples. Gene ontology functional annotation of selected DEGs was performed through DAVID online database to clarify the biological characteristics of DEGs. The main pathways of DEGs were obtained by enrichment analysis using Kyoto gene and genomic encyclopedia method. Based on the STRING database, the DEGs protein-protein interaction network was constructed by using CytoScape software, and the key genes were screened by GEPIA2 database to verify the expression at the cell level. Results: A total of 238 DEGs were screened from GSE38666, GSE40595 and GSE54388 datasets, of which 168 DEGs were up-regulated and 70 DEGs were down-regulated. The co-expressed DEGs were mainly enriched in biological functions such as mitotic nuclear division, spindle, chromosomal region and DNA helicase activity in ovarian cancer. They were mainly involved in biological processes such as cell cycle, DNA replication, oxidative phosphorylation and biosynthesis of amino acids, thereby affecting the occurrence and development of ovarian cancer. Six genes were highly expressed and associated with the development of ovarian cancer, including IFI27, EPCAM, CXCR4, PEA15, CLDN3 and CAPG. Cell verification showed that the mRNA expression of the six genes in ovarian cancer cells was higher than that in normal ovarian cells (P<0.05), which was consistent with the previous screening results. Conclusion: Multi-chip integrated bioinformatics is an effective method to find ovarian cancer target genes. IFI27, EPCAM, CXCR4, PEA15, CLDN3 and CAPG are highly correlated with the occurrence and development of ovarian cancer, which can be used as target genes for ovarian cancer research.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Abnormal expression of TGFβ1 in acute myeloid leukemia and its regulation effect on leukemia cells

    CHEN Wen-tingHUANG YingPAN Yan-pingWANG Shu-wen...
    47-53页
    查看更多>>摘要：Objective: To explore the level of acute myeloid leukemia (COX-2), transforming growth factor β1 (TGFβ1), basic fibroblast growth factor (bFGF), and vascular endothelial growth factor (VEGF) in acute myeloid leukemia (AML) patients and to investigate the role of TGFβ1 on the proliferation, apoptosis and cycle of AML cells, providing new targets and research bases for the treatment and prognostic evaluation of AML. Methods: Peripheral blood was collected from 80 AML patients and 60 normal people. The levels of COX-2, TGFβ1, bFGF and VEGF in peripheral blood mononuclear cells and serum were determined by using quantitative polymerase chain reaction and enzyme linked immunosorbent assay, respectively. The proliferation, apoptosis and cycle of AML cells affected by overexpression and silencing of TGFβ1 was detected using cell counting kit-8 and flow cytometry. Results: The expression of COX-2, TGFβ1, bFGF and VEGF increased significantly in peripheral blood mononuclear cells and serum in AML patients. TGFβ1 promoted AML cell proliferation, inhibited its apoptosis, and increased stage G2 cell proportion. Conclusion: COX-2, TGFβ1, bFGF and VEGF play important roles in the progression of AML. TGFβ1 is a new potential biomarker for the diagnosis and treatment of AML.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    National patent Chinese herbal compound for parkinson's disease based on data mining and network pharmacology analysis

    PAN YuWANG LiangWANG PengYANG Ming-hui...
    54-61页
    查看更多>>摘要：Objective: To analyze the dosing pattern and mechanism of herbal compound patents for the treatment of Parkinson's disease using data mining and network pharmacology methods, and to provide ideas for the clinical use of Parkinson's disease and new drug development. Methods: The Chinese herbal medicine compound prescriptions for Parkinson's disease built up to May 31, 2022 by searching the official website of patent publication notice. An Excel table was built, and after term normalization of the included compound prescriptions,Excel and IBM SPSS Modeler 18.0 were used for data mining such as frequency statistics. We also applied network pharmacology methods to study HF drugs, using TCMSP and TCM database@Taiwan to obtain drug components and using TCMSP platform and Swiss ADME screening, collecting targets through UniProt and Swiss Target Prediction platform; obtaining PD disease from databases such as GeneCards The targets were obtained from GeneCards and other databases, drug-disease target intersections were obtained, and "drug-disease-target" networks were created using Cytoscape 3.8 software, and GO and KEGG enrichment analyses were performed using the Metascapep platform. Results: A total of 113 patented Chinese medicine recipes were included, involving 394 drugs, among which Tianma, Angelica and Bai Shao were the most commonly used drugs. A total of 442 drug targets, 4884 disease targets and 324 drug-disease common targets were obtained. Conclusion: The study found that the medicinal properties of the patented compound Chinese medicine for Parkinson's disease are mainly warm and cold, the taste of the medicine is mainly sweet and bitter, the normalizing meridian is mainly liver, heart and spleen meridians, and the treatment method is mainly to strengthen the liver and kidney, nourish the qi and blood, and extinguish the wind and dredge the luo. The core ingredients include 4-ethoxymethylphenyl-4'-hydroxyl, palmitic acid, lignan, etc.; the main action targets are PTGS2, PTGS1, SCN5A, etc.; involved in PI3K-Akt, cAMP and other signaling pathways. The development of relevant compound can be based on clinical symptoms, appropriate tailoring, and flexible use of such drugs, in order to obtain the best therapeutic effect.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Efficacy and safety of awn needle in the treatment of lumbar disc herniation: A meta-analysis

    CUI Hai-lingLI Yan-jvCHEN Xing-shengWANG Min-jun...
    62-70页
    查看更多>>摘要：Objective: To evaluate the intervention effect of awn needle in the treatment of lumbar disc herniation through meta-analysis. Methods: In CNKI, Wanfang Data, PubMed, VIP and other databases, we searched the clinical observation studies on the treatment of patients with lumbar disc herniation with awn needle. The methodological quality of the included studies was evaluated using Cochrane 5.1.0 bias risk assessment criteria and Jadad scale. Revman 5.3 software was used for meta-analysis. Results: A total of 14 randomized controlled trials involving 1186 patients were included, with 595 in the awn needle treatment group and 591 in the control group. Meta-analysis showed that the awn needle treatment group was better than that of the control group in the total effective rate[RR=1.17; 95％CI(1.12,1.22); Z=6.66; P<0.00001]; the awn needle treatment group was better than the control group in ameliorating VAS score [WMD=-1.07, 95％CI(-1.19,-0.95), Z=17.87, P<0.00001]; the awn needle treatment group was better than the control group in ameliorating JOA score [WMD=3.18, 95％CI(2.64,3.71), Z=11.57, P<0.00001]; the awn needle treatment group was better than the control group in the amelioration of ADL score [SMD =2.44, 95％CI(0.54, 4.35), Z=2.52, P=0.01]; the awn needle treatment group was better than the control group in the improvement of SLRT angle [WMD=12.71, 95％CI(3.21, 22.21), Z=2.62, P=0.009]. Conclusion: Awn needle treatment can improve the total effective rate of patients with lumbar disc herniation,relieve the pain of patients, improve the straight leg elevation angle of patients, alleviate the disease development, relieve the symptoms, and improve the quality of life of patients. It is worthy of clinical application.
      原文链接:
    • NETL
    • NSTL
    • 万方数据