首页|Mechanism of calcium hydroxide inhibiting methicillin resistant Staphylococcus aureus

Mechanism of calcium hydroxide inhibiting methicillin resistant Staphylococcus aureus

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Objective:To investigate the inhibitory effect of calcium hydroxide on methicillin-resistant Staphylococcus aureus and the related inhibition mechanism. Methods: To determine the minimum inhibitory concentration of calcium hydroxide using microplate dilution method; to compare the effects of calcium hydroxide at 8MIC, MIC, 1/4MIC and 0 concentrations on MRSA using growth curve method; to determine the effects of calcium hydroxide on the cell membrane of methicillin-resistant Staphylococcus aureus using calcium xanthophyll and propidium iodide fluorescence staining The effect of calcium hydroxide on the morphology of methicillin-resistant Staphylococcus aureus was observed by scanning electron microscopy; the inhibition mechanism of calcium hydroxide on MRSA was investigated by sodium dodecyl sulfate polyacrylamide gel electrophoresis of protein bands. Results: The MIC of calcium hydroxide on MRSA was 3.125 mg/mL; the fluorescence intensity showed significant changes after co-culture of calcium hydroxide with bacteria; MRSA appeared to be significantly crumpled and broken in the presence of calcium hydroxide; the SDS-PAGE experimental bands indicated that the protein inside the bacteria decreased accordingly with the increase of calcium hydroxide concentration. Conclusion: Calcium hydroxide has a significant inhibitory effect on the growth of MRSA, and its bactericidal mechanism may be related to the destruction of bacterial body structure.

Calcium hydroxideMethicillin-resistant staphylococcusMRSABacteriostasisAntibacterial mechanismCytomembrane

Zheng-Dong Guo、Yang-Yang Bian、Xiao-Qian Liu、Dong Wang、Si-Yuan Zhang、Jian Yang、Lei Peng

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Trauma medical center of the First Affiliated Hospital of Hainan Medical College,Haikou,Hainan 570216,China

Key Laboratory of Emergency and Trauma Ministry of Education,Hainan Medical University,Haikou,Hainan 570216,China

Key Laboratory of Hainan Trauma and Disaster Rescue,The First Affiliated Hospital of Hainan Medical University,Haikou,Hainan 570216,China

国家自然科学基金

82060347

2022

海南医科大学学报(英文版)

海南医科大学学报(英文版)

ISSN：
年,卷(期)：2022.28(5)
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