Mechanism of hesperidin improving myocardial ischemia/reperfusion injury in type 2 diabetic rats through SIRT1/Nrf2/HO-1 signaling pathway

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外文摘要：Objective: To observe the protective effect of hesperidin on myocardial ischemia/ reperfusion injury in type 2 diabetes mellitus and its effect on SIRT1/Nrf2/HO-1 signaling pathway. Methods: 50 Sprague-Dawley (SD) rats were randomly assigned to the normal control group (NC), model group, ischemia-reperfusion group (IR), hesperidin group, SIRT1 inhibitor group and hesperidin plus SIRT1 inhibitor group. In addition to NC, the rats in the remaining groups were replicated by intraperitoneal of high-fat diet combined with injection of streptozotocin for type 2 diabetic rats. After then, the myocardial ischemia/reperfusion injury (MIRI) rat model was established by LAd for 30 minutes with 2 hours reperfusion. He staining was used to observe the pathological changes of myocardial tissue, and the levels of serum LDH, CK-MB and SOD, GSH and MDA in myocardial tissue were detected by kit methods, and the expression abundance of related proteins in 4-HNE and SIRT1/Nrf2/HO-1 signal pathway were detected by immunohistochemistry and Western blot; Results: Hesperidin could significantly inhibit cardiomyocyte necrosis and inflammatory cell infiltration, reduce LDH activity, CK-MB and MDA level, and increase SOD activity, GSH and 4-HNE level, the differences were statistically significant when compared with IR group (P<0.01). In addition, compared with the ischemia-reperfusion group, the expressions of SIRT1, Nrf2 and HO-1 proteins in hesperidin group were significantly up-regulated, the differences were statistically significant (P<0.01); Conclusion: Hesperidin inhibits oxidative stress by activating SIRT1/Nrf2/HO-1 signaling pathway, and play a protective effect of myocardial ischemia reperfusion injury in diabetic rats.

外文关键词：

HesperidinType 2 diabetes mellitusIschemia/reperfusionMyocardial injurySIRT1/Nrf2/HO-1 signaling pathway

作者：

Zhen-Wang Ma、De-You Jiang、Bing-Cheng Hu、Xing-Xing Yuan、Shao-Jie Cai、Jing Guo

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作者单位：

Heilongjiang University of traditional Chinese medicine,Harbin 150040,China

Heilongjiang Academy of traditional Chinese medicine,Harbin 150006,China

基金：

Construction Project of Traditional Chinese Medicine Academic Genre Inheritance Studio of the State Administration of TraditionaConstruction Project of National Famous and old Traditional Chinese Medicine Expert Inheritance Studio of the State Administrati

项目编号：

LPGZS2012-14

出版年：

2022

海南医科大学学报(英文版)

ISSN：

年,卷(期)：2022.28(8)

参考文献量15