首页|Chlorogenic acid modulates glucose and lipid metabolisms via AMPK activation in HepG2 cells and shows its anti-hyperglycemic effect on streptozocin-induced diabetic mice

Chlorogenic acid modulates glucose and lipid metabolisms via AMPK activation in HepG2 cells and shows its anti-hyperglycemic effect on streptozocin-induced diabetic mice

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Objective: In this study, we focus on the hypoglycemic effects of chlorogenic acid (CGA) in vitro and in vivo and its mechanism base on regulate glucose and lipid metabolism via AMPK activation. Methods: The cytotoxicity, glucose consumption and intracellular triglyceride assay were been detected by commercial kits. The western blots were used to detection the associated protein levels after CGA treatment, and the inhibiter blocking experiments were also be done. In vivo experiment, the fasting blood-glucose, lipid metabolism, liver function, insulin resistance, glucose tolerance, and pathological change were assessed on streptozocin induced diabetic mice. Results: We found that CGA exhibited no cytotoxicity at concentrations of 100μM, it caused a significant increasing of glucose consumption and reducing of the PA-BSA induced intracellular TG level on HepG2 cells at 50μM and 100μM treatment, CGA exhibited up-regulating the level of p-AMPK (Thr172) and p-ACC (Ser79) in dose-dependent manners in vitro and in vivo. The stimulating activities of CGA on AMPK were completely blocked by compound c (CC) on HepG2 cells. And the efficacies of CGA on glucose consumption and intracellular TG accumulation were also completely blocked by CC pretreatment. The CGA also exhibited potent anti-diabetic effects with hypoglycemic activity, improve insulin resistance and glucose tolerance, regulate glucose and lipid metabolism and protect the liver function in vivo. Conclusion: Our results suggested that CGA can regulate glucose and lipid metabolism by AMPK activation, and exhibit potent anti-hyperglycemic effect in streptozocin induced diabetes mice, and may be used as a potential effective anti-diabetes drug.

Chlorogenic acidGlucose and lipid metabolismAnti-diabetes activityAMPK

Hong-Ji Yao、Zhao-Yi Xue、Rui-Qi Wang、Cheng-Zuo Jiang、Jing-Yan Xiong、Zhi-Xuan Xia、Qiang Liu、Qi-Bing Li、Yong Zhang

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Department of Pharmacology,Hainan Medical University,Haikou 571199,China

Research Center of Pharmaceutical Engineering Technology,Harbin University of Commerce,Harbin 150076,China

Natural Science Foundation for Youth of Hainan ProvinceScientific Research Project of Hainan Colleges and UniversitiesInnovation and Entrepreneurship Training Planning Project for College StudentsInnovation and Entrepreneurship Training Planning Project for College Students

819QN226Hnky2019ZD-32202011810010X20201181058

2022

海南医科大学学报(英文版)

海南医科大学学报(英文版)

ISSN：
年,卷(期)：2022.28(9)
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