首页|Design and characterization of a bi-functional bybrid antibacterial peptide LLM against Pseudomonas aeruginosa

Design and characterization of a bi-functional bybrid antibacterial peptide LLM against Pseudomonas aeruginosa

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
  • 维普
Objective: To design a bifunctional antimicrobial peptide with high antibacterial activity and endotoxin neutralization against P.aeruginosa and explore its bactericidal properties. Methods: The neutralizing endotoxin peptides and antimicrobial peptide against P.aeruginosa were connected by a GGGS-linker to get a bi-functional bybrid peptide. Its structural parameter was tested by EMBOSS software. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and bactericidal kinetics against P.aeruginosa were determined. Its effect on endotoxin neutralization and hemolysis were also evaluated. Results: We designed and obtained a bybrid peptide LLM, which carried +8 positive charge with high activity against P.aeruginosa and neutralizing endotoxin. The MIC of LLM against P.aeruginosa CMCC10104 and P.aeruginosa ATCC 9027 were 2 and 4 μmol/L, and MBC/MIC equal 1. LLM showed rapid anti- P.aeruginosa effects and significantly neutralized the endotoxin released, and not exhibited hemolysis as high as 115 μmol/L (400 μg/mL).Conclusion: The MICs of LLM against P.aeruginosa were 2～4 μmol/L, it showed significant activity of anti-P. aeruginosa and neutralizing endotoxin, and could kill bacteria quickly,and did not show significant hemolytic under 115 μmol/L.

Hybrid antimicrobial peptidesPseudomonas aeruginosaEndotoxin neutralizationBactericidalHemolytic

ZHANG Ze-jin、YAO Hong-ji、XUE Zhao-yi、ZHAN Yu-tong、GUO Ying-jie、WU Meng、LIN Ke-xin、XIONG Jing-yan、ZHANG Yong

展开 >

The First Clinical College of Hainan Medical University,Haikou 571199,China

School of Basic Medicine and Life Science,Hainan Medical University,Haikou 571199,China

Scientific Research Project of Hainan Colleges and UniversitiesInnovation and Entrepreneurship Training Planning Project for College Students

Hnky2019ZD-32S202011810018

2022

海南医科大学学报(英文版)

海南医科大学学报(英文版)

ISSN：
年,卷(期)：2022.28(21)
  • 1